Imperial College London

DrIsabelGarcia Perez

Faculty of MedicineDepartment of Surgery & Cancer

Lecturer in Precision & Systems Medicine
 
 
 
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i.garcia-perez

 
 
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101Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
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37 results found

Chambers ES, Byrne CS, Morrison DJ, Murphy KG, Preston T, Tedford C, Garcia-Perez I, Fountana S, Serrano-Contreras JI, Holmes E, Reynolds CJ, Roberts JF, Boyton RJ, Altmann DM, McDonald JAK, Marchesi JR, Akbar AN, Riddell NE, Wallis GA, Frost GSet al., 2019, Dietary supplementation with inulin-propionate ester or inulin improves insulin sensitivity in adults with overweight and obesity with distinct effects on the gut microbiota, plasma metabolome and systemic inflammatory responses: a randomised cross-over trial., Gut

OBJECTIVE: To investigate the underlying mechanisms behind changes in glucose homeostasis with delivery of propionate to the human colon by comprehensive and coordinated analysis of gut bacterial composition, plasma metabolome and immune responses. DESIGN: Twelve non-diabetic adults with overweight and obesity received 20 g/day of inulin-propionate ester (IPE), designed to selectively deliver propionate to the colon, a high-fermentable fibre control (inulin) and a low-fermentable fibre control (cellulose) in a randomised, double-blind, placebo-controlled, cross-over design. Outcome measurements of metabolic responses, inflammatory markers and gut bacterial composition were analysed at the end of each 42-day supplementation period. RESULTS: Both IPE and inulin supplementation improved insulin resistance compared with cellulose supplementation, measured by homeostatic model assessment 2 (mean±SEM 1.23±0.17 IPE vs 1.59±0.17 cellulose, p=0.001; 1.17±0.15 inulin vs 1.59±0.17 cellulose, p=0.009), with no differences between IPE and inulin (p=0.272). Fasting insulin was only associated positively with plasma tyrosine and negatively with plasma glycine following inulin supplementation. IPE supplementation decreased proinflammatory interleukin-8 levels compared with cellulose, while inulin had no impact on the systemic inflammatory markers studied. Inulin promoted changes in gut bacterial populations at the class level (increased Actinobacteria and decreased Clostridia) and order level (decreased Clostridiales) compared with cellulose, with small differences at the species level observed between IPE and cellulose. CONCLUSION: These data demonstrate a distinctive physiological impact of raising colonic propionate delivery in humans, as improvements in insulin sensitivity promoted by IPE and inulin were accompanied with different effects on the plasma metabolome, gut bacterial populations and markers of systemic in

JOURNAL ARTICLE

Byrne CS, Preston T, Brignardello J, Garcia-Perez I, Holmes E, Frost GS, Morrison DJet al., 2018, The effect of L-rhamnose on intestinal transit time, short chain fatty acids and appetite regulation: a pilot human study using combined (CO2)-C-13/H-2 breath tests, JOURNAL OF BREATH RESEARCH, Vol: 12, ISSN: 1752-7155

JOURNAL ARTICLE

Cronin O, Barton W, Skuse P, Penney NC, Garcia-Perez I, Murphy EF, Woods T, Nugent H, Fanning A, Melgar S, Falvey EC, Holmes E, Cotter PD, O'Sullivan O, Molloy MG, Shanahan Fet al., 2018, A Prospective Metagenomic and Metabolomic Analysis of the Impact of Exercise and/or Whey Protein Supplementation on the Gut Microbiome of Sedentary Adults, MSYSTEMS, Vol: 3, ISSN: 2379-5077

JOURNAL ARTICLE

Posma JM, Garcia-Perez I, Ebbels TMD, Lindon JC, Stamler J, Elliott P, Holmes E, Nicholson JKet al., 2018, Optimized Phenotypic Biomarker Discovery and Confounder Elimination via Covariate-Adjusted Projection to Latent Structures from Metabolic Spectroscopy Data, JOURNAL OF PROTEOME RESEARCH, Vol: 17, Pages: 1586-1595, ISSN: 1535-3893

JOURNAL ARTICLE

Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD, O'Sullivan Oet al., 2018, The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level, GUT, Vol: 67, Pages: 625-633, ISSN: 0017-5749

JOURNAL ARTICLE

Garcia Perez I, Posma JM, Gibson R, Chambers ES, Nicholson JK, Holmes E, Frost Get al., 2017, Modernizing dietary assessment, IUNS 21st ICN International Congress of Nutrition, Publisher: Karger Publishers, Pages: 286-287, ISSN: 1421-9697

CONFERENCE PAPER

Swann JR, Garcia-Perez I, Braniste V, Wilson ID, Sidaway JE, Nicholson JK, Pettersson S, Holmes Eet al., 2017, Application of H-1 NMR spectroscopy to the metabolic phenotyping of rodent brain extracts: A metabonomic study of gut microbial influence on host brain metabolism, JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, Vol: 143, Pages: 141-146, ISSN: 0731-7085

JOURNAL ARTICLE

Posma JM, Garcia-Perez I, Heaton JC, Burdisso P, Mathers JC, Draper J, Lewis M, Lindon JC, Frost G, Holmes E, Nicholson JKet al., 2017, Integrated Analytical and Statistical Two-Dimensional Spectroscopy Strategy for Metabolite Identification: Application to Dietary Biomarkers, ANALYTICAL CHEMISTRY, Vol: 89, Pages: 3300-3309, ISSN: 0003-2700

JOURNAL ARTICLE

Garcia-Perez I, Posma JM, Gibson R, Chambers ES, Hansen TH, Vestergaard H, Hansen T, Beckmann M, Pedersen O, Elliott P, Stamler J, Nicholson JK, Draper J, Mathers JC, Holmes E, Frost Get al., 2017, Objective assessment of dietary patterns by use of metabolic phenotyping: a randomised, controlled, crossover trial, LANCET DIABETES & ENDOCRINOLOGY, Vol: 5, Pages: 184-195, ISSN: 2213-8587

JOURNAL ARTICLE

Brignardello J, Holmes E, Garcia-Perez I, 2017, Metabolic Phenotyping of Diet and Dietary Intake., Pages: 231-270

Nutrition provides the building blocks for growth, repair, and maintenance of the body and is key to maintaining health. Exposure to fast foods, mass production of dietary components, and wider importation of goods have challenged the balance between diet and health in recent decades, and both scientists and clinicians struggle to characterize the relationship between this changing dietary landscape and human metabolism with its consequent impact on health. Metabolic phenotyping of foods, using high-density data-generating technologies to profile the biochemical composition of foods, meals, and human samples (pre- and postfood intake), can be used to map the complex interaction between the diet and human metabolism and also to assess food quality and safety. Here, we outline some of the techniques currently used for metabolic phenotyping and describe key applications in the food sciences, ending with a broad outlook at some of the newer technologies in the field with a view to exploring their potential to address some of the critical challenges in nutritional science.

BOOK CHAPTER

Kaluarachchi MR, Boulange CL, Garcia-Perez I, Lindon JC, Minet EFet al., 2016, Multiplatform serum metabolic phenotyping combined with pathway mapping to identify biochemical differences in smokers, BIOANALYSIS, Vol: 8, Pages: 2023-2043, ISSN: 1757-6180

JOURNAL ARTICLE

Serrano-Contreras JI, Garcia-Perez I, Melendez-Camargo ME, Gerardo Zepeda Let al., 2016, NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat, JOURNAL OF PROTEOME RESEARCH, Vol: 15, Pages: 3241-3254, ISSN: 1535-3893

JOURNAL ARTICLE

Serrano-Contreras JI, Garcia-Perez I, Melendez-Camargo ME, Zepeda-Vallejo LGet al., 2016, NMR-based metabonomic analysis of normal rat urine and faeces in response to (+/-)-venlafaxine treatment, JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, Vol: 123, Pages: 82-92, ISSN: 0731-7085

JOURNAL ARTICLE

Garcia-Perez I, Posma JM, Chambers ES, Nicholson JK, Mathers JC, Beckmann M, Draper J, Holmes E, Frost Get al., 2016, An Analytical Pipeline for Quantitative Characterization of Dietary Intake: Application To Assess Grape Intake, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Vol: 64, Pages: 2423-2431, ISSN: 0021-8561

JOURNAL ARTICLE

Stradling C, Thomas GN, Hemming K, Frost G, Garcia-Perez I, Redwood S, Taheri Set al., 2016, Randomised controlled pilot study to assess the feasibility of a Mediterranean Portfolio dietary intervention for cardiovascular risk reduction in HIV dyslipidaemia: a study protocol, BMJ OPEN, Vol: 6, ISSN: 2044-6055

JOURNAL ARTICLE

Lloyd AJ, Zubair H, Willis ND, Wilson T, Xie L, Tailliart K, Chambers ES, Garcia-Perez I, Holmes E, Frost G, Mathers JC, Beckmann M, Draper Jet al., 2016, Quantification of dietary biomarkers in spot urine samples reflects the intake of foods of UK high public health importance, Publisher: CAMBRIDGE UNIV PRESS, Pages: E248-E248, ISSN: 0029-6651

CONFERENCE PAPER

Andreas NJ, Hyde MJ, Gomez-Romero M, Angeles Lopez-Gonzalvez M, Villasenor A, Wijeyesekera A, Barbas C, Modi N, Holmes E, Garcia-Perez Iet al., 2015, Multiplatform characterization of dynamic changes in breast milk during lactation, ELECTROPHORESIS, Vol: 36, Pages: 2269-2285, ISSN: 0173-0835

JOURNAL ARTICLE

Elliott P, Posma JM, Chan Q, Garcia-Perez I, Wijeyesekera A, Bictash M, Ebbels TMD, Ueshima H, Zhao L, van Horn L, Daviglus M, Stamler J, Holmes E, Nicholson JKet al., 2015, Urinary metabolic signatures of human adiposity, SCIENCE TRANSLATIONAL MEDICINE, Vol: 7, ISSN: 1946-6234

JOURNAL ARTICLE

Hall AM, Vilasi A, Garcia-Perez I, Lapsley M, Alston CL, Pitceathly RDS, McFarland R, Schaefer AM, Turnbull DM, Beaumont NJ, Hsuan JJ, Cutillas PR, Lindon JC, Holmes E, Unwin RJ, Taylor RW, Gorman GS, Rahman S, Hanna MGet al., 2015, The urinary proteome and metabonome differ from normal in adults with mitochondrial disease, KIDNEY INTERNATIONAL, Vol: 87, Pages: 610-622, ISSN: 0085-2538

JOURNAL ARTICLE

Willis ND, Dodds SE, Lloyd AJ, Xie L, Chambers ES, Garcia-Perez I, Frost G, Beckmann M, Draper J, Mathers JCet al., 2015, Dietary compliance in a human intervention study investigating the impact of specific foods on urinary metabolites, PROCEEDINGS OF THE NUTRITION SOCIETY, Vol: 74, Pages: E127-E127, ISSN: 0029-6651

JOURNAL ARTICLE

Villasenor A, Garcia-Perez I, Garcia A, Posma JM, Fernandez-Lopez M, Nicholas AJ, Modi N, Holmes E, Barbas Cet al., 2014, Breast Milk Metabolome Characterization in a Single-Phase Extraction, Multiplatform Analytical Approach, ANALYTICAL CHEMISTRY, Vol: 86, Pages: 8245-8252, ISSN: 0003-2700

JOURNAL ARTICLE

Garcia-Perez I, Lindon JC, Minet E, 2014, Application of CE-MS to a metabonomics study of human urine from cigarette smokers and non-smokers, BIOANALYSIS, Vol: 6, Pages: 2733-2749, ISSN: 1757-6180

JOURNAL ARTICLE

Stamler J, Brown IJ, Yap IKS, Chan Q, Wijeyesekera A, Garcia-Perez I, Chadeau-Hyam M, Ebbels TMD, De Iorio M, Posma J, Daviglus ML, Carnethon M, Holmes E, Nicholson JK, Elliott Pet al., 2013, Dietary and Urinary Metabonomic Factors Possibly Accounting for Higher Blood Pressure of Black Compared With White Americans Results of International Collaborative Study on Macro-/Micronutrients and Blood Pressure, HYPERTENSION, Vol: 62, Pages: 1074-1080, ISSN: 0194-911X

JOURNAL ARTICLE

Liebeke M, Garcia-Perez I, Anderson CJ, Lawlor AJ, Bennett MH, Morris CA, Kille P, Svendsen C, Spurgeon DJ, Bundy JGet al., 2013, Earthworms Produce phytochelatins in Response to Arsenic, PLOS ONE, Vol: 8, ISSN: 1932-6203

JOURNAL ARTICLE

Ismail NA, Posma JM, Frost G, Holmes E, Garcia-Perez Iet al., 2013, The role of metabonomics as a tool for augmenting nutritional information in epidemiological studies, ELECTROPHORESIS, Vol: 34, Pages: 2776-2786, ISSN: 0173-0835

JOURNAL ARTICLE

Posma JM, Garcia-Perez I, De Iorio M, Lindon JC, Elliott P, Holmes E, Ebbels TMD, Nicholson JKet al., 2012, Subset Optimization by Reference Matching (STORM): An Optimized Statistical Approach for Recovery of Metabolic Biomarker Structural Information from H-1 NMR Spectra of Biofluids, ANALYTICAL CHEMISTRY, Vol: 84, Pages: 10694-10701, ISSN: 0003-2700

JOURNAL ARTICLE

Alves AC, Li JV, Garcia-Perez I, Sands C, Barbas C, Holmes E, Ebbels TMDet al., 2012, Characterization of data analysis methods for information recovery from metabolic H-1 NMR spectra using artificial complex mixtures, METABOLOMICS, Vol: 8, Pages: 1170-1180, ISSN: 1573-3882

JOURNAL ARTICLE

Garcia-Perez I, Villasenor A, Wijeyesekera A, Posma JM, Jiang Z, Stamler J, Aronson P, Unwin R, Barbas C, Elliott P, Nicholson J, Holmes Eet al., 2012, Urinary Metabolic Phenotyping the slc26a6 (Chloride-Oxalate Exchanger) Null Mouse Model, JOURNAL OF PROTEOME RESEARCH, Vol: 11, Pages: 4425-4435, ISSN: 1535-3893

JOURNAL ARTICLE

Ernst A, Ma D, Garcia-Perez I, Tsang TM, Kluge W, Schwarz E, Guest PC, Holmes E, Sarnyai Z, Bahn Set al., 2012, Molecular Validation of the Acute Phencyclidine Rat Model for Schizophrenia: Identification of Translational Changes in Energy Metabolism and Neurotransmission, JOURNAL OF PROTEOME RESEARCH, Vol: 11, Pages: 3704-3714, ISSN: 1535-3893

JOURNAL ARTICLE

Yap IKS, Brown IJ, Chan Q, Wijeyesekera A, Garcia-Perez I, Bictash M, Loo RL, Chadeau-Hyam M, Ebbeis T, De Iorio M, Maibaum E, Zhao L, Kesteloot H, Daviglus ML, Stamler J, Nicholson JK, Elliott P, Holmes Eet al., 2010, Metabolome-Wide Association Study Identifies Multiple Biomarkers that Discriminate North and South Chinese Populations at Differing Risks of Cardiovascular Disease INTERMAP Study, JOURNAL OF PROTEOME RESEARCH, Vol: 9, Pages: 6647-6654, ISSN: 1535-3893

JOURNAL ARTICLE

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