Imperial College London
Alternate

DrIsabelGarcia Perez

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Precision and Systems Medicine
 
 
 
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Contact

 

i.garcia-perez

 
 
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Location

 

101Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lahiri:2019:10.1126/scitranslmed.aan5662,
author = {Lahiri, S and Kim, H and Garcia-Perez, I and Reza, MM and Martin, KA and Kundu, P and Cox, LM and Selkrig, J and Posma, JM and Zhang, H and Padmanabhan, P and Moret, C and Gulyás, B and Blaser, MJ and Auwerx, J and Holmes, E and Nicholson, J and Wahli, W and Pettersson, S},
doi = {10.1126/scitranslmed.aan5662},
journal = {Science Translational Medicine},
title = {The gut microbiota influences skeletal muscle mass and function in mice},
url = {http://dx.doi.org/10.1126/scitranslmed.aan5662},
volume = {11},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The functional interactions between the gut microbiota and the host are important for host physiology, homeostasis, and sustained health. We compared the skeletal muscle of germ-free mice that lacked a gut microbiota to the skeletal muscle of pathogen-free mice that had a gut microbiota. Compared to pathogen-free mouse skeletal muscle, germ-free mouse skeletal muscle showed atrophy, decreased expression of insulin-like growth factor 1, and reduced transcription of genes associated with skeletal muscle growth and mitochondrial function. Nuclear magnetic resonance spectrometry analysis of skeletal muscle, liver, and serum from germ-free mice revealed multiple changes in the amounts of amino acids, including glycine and alanine, compared to pathogen-free mice. Germ-free mice also showed reduced serum choline, the precursor of acetylcholine, the key neurotransmitter that signals between muscle and nerve at neuromuscular junctions. Reduced expression of genes encoding Rapsyn and Lrp4, two proteins important for neuromuscular junction assembly and function, was also observed in skeletal muscle from germ-free mice compared to pathogen-free mice. Transplanting the gut microbiota from pathogen-free mice into germ-free mice resulted in an increase in skeletal muscle mass, a reduction in muscle atrophy markers, improved oxidative metabolic capacity of the muscle, and elevated expression of the neuromuscular junction assembly genes <jats:italic>Rapsyn</jats:italic> and <jats:italic>Lrp4</jats:italic>. Treating germ-free mice with short-chain fatty acids (microbial metabolites) partly reversed skeletal muscle impairments. Our results suggest a role for the gut microbiota in regulating skeletal muscle mass and function in mice.</jats:p>
AU  - Lahiri,S
AU  - Kim,H
AU  - Garcia-Perez,I
AU  - Reza,MM
AU  - Martin,KA
AU  - Kundu,P
AU  - Cox,LM
AU  - Selkrig,J
AU  - Posma,JM
AU  - Zhang,H
AU  - Padmanabhan,P
AU  - Moret,C
AU  - Gulyás,B
AU  - Blaser,MJ
AU  - Auwerx,J
AU  - Holmes,E
AU  - Nicholson,J
AU  - Wahli,W
AU  - Pettersson,S
DO  - 10.1126/scitranslmed.aan5662
PY  - 2019///
SN  - 1946-6234
TI  - The gut microbiota influences skeletal muscle mass and function in mice
T2  - Science Translational Medicine
UR  - http://dx.doi.org/10.1126/scitranslmed.aan5662
UR  - https://stm.sciencemag.org/content/11/502/eaan5662
UR  - http://hdl.handle.net/10044/1/71970
VL  - 11
ER  -
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