Imperial College London
Alternate

DrIsabelGarcia Perez

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Precision and Systems Medicine
 
 
 
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Contact

 

i.garcia-perez

 
 
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Location

 

101Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Karampatsas:2022:10.1097/MD.0000000000031419,
author = {Karampatsas, K and Faal, A and Jaiteh, M and Garcia-Perez, I and Aller, S and Shaw, A and Kopytek, A and Witney, A and Le, Doare K},
doi = {10.1097/MD.0000000000031419},
journal = {Medicine},
pages = {1--10},
title = {Gastrointestinal, vaginal, nasopharyngeal, and breast milk microbiota profiles and breast milk metabolomic changes in Gambian infants over the first two months of lactation: a prospective cohort study},
url = {http://dx.doi.org/10.1097/MD.0000000000031419},
volume = {101},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: Microbiota composition in breast milk affects intestinal and respiratory microbiota colonization and the mucosal immune system's development in infants. The metabolomic content of breast milk is thought to interact with the microbiota and may influence developing infant immunity.Methods: 107 Gambian mothers and their healthy, vaginally delivered, exclusively breastfed infants were included in our study. We analyzed 32 breast milk samples, 51 maternal rectovaginal swabs and 30 infants' rectal swabs at birth. We also analyzed 9 breast milk samples and 18 infants' nasopharyngeal swabs 60 days post-delivery. We used 16S rRNA genesequencing to determine the microbiota composition. Metabolomic profiling analysis was performed on colostrum and mature breast milk samples using a multiplatform approach combining 1-H Nuclear Magnetic Resonance Spectroscopy and Gas Chromatography-Mass Spectrometry.Results: Bacterial communities were distinct in composition and diversity across different sample types. Breast milk composition changed over the first 60 days of lactation. α-1,4- and α-1,3-fucosylated human milk oligosaccharides, and other 33 key metabolites in breast milk(monosaccharides, sugar alcohols and fatty acids) increased between birth and day of 60 life.Conclusions: This study's results indicate that infant gut and respiratory microbiota are unique bacterial communities, distinct from maternal gut and breast milk, respectively. Breast milk microbiota composition and metabolomic profile change throughout lactation. These changesmay contribute to the infant's immunological, metabolic, and neurological development and could consist the basis for future interventions to correct disrupted early life microbial colonization.
AU  - Karampatsas,K
AU  - Faal,A
AU  - Jaiteh,M
AU  - Garcia-Perez,I
AU  - Aller,S
AU  - Shaw,A
AU  - Kopytek,A
AU  - Witney,A
AU  - Le,Doare K
DO  - 10.1097/MD.0000000000031419
EP  - 10
PY  - 2022///
SN  - 0025-7974
SP  - 1
TI  - Gastrointestinal, vaginal, nasopharyngeal, and breast milk microbiota profiles and breast milk metabolomic changes in Gambian infants over the first two months of lactation: a prospective cohort study
T2  - Medicine
UR  - http://dx.doi.org/10.1097/MD.0000000000031419
UR  - https://journals.lww.com/md-journal/Fulltext/2022/11180/Gastrointestinal,_vaginal,_nasopharyngeal,_and.31.aspx
UR  - http://hdl.handle.net/10044/1/100295
VL  - 101
ER  -
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