Imperial College London
Alternate

DrIsabelGarcia Perez

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Precision and Systems Medicine
 
 
 
//

Contact

 

i.garcia-perez

 
 
//

Location

 

101Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Serrano-Contreras:2016:10.1021/acs.jproteome.6b00433,
author = {Serrano-Contreras, JI and Garcia-Perez, I and Melendez-Camargo, ME and Gerardo, Zepeda L},
doi = {10.1021/acs.jproteome.6b00433},
journal = {Journal of Proteome Research},
pages = {3241--3254},
title = {NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat},
url = {http://dx.doi.org/10.1021/acs.jproteome.6b00433},
volume = {15},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via adaptive mechanisms. After refeeding, when food is available, other adaptive processes are linked to energy balance. However, less has been reported about the physiological mechanisms present as a result of these conditions, considering the rat as a supraorganism. Metabolic profiling using 1H nuclear magnetic resonance spectroscopy was used to characterize the physiological metabolic differences in urine specimens collected under starved, refed, and recovered conditions. In addition, because starvation induced lack of faecal production and not all animals produced faeces during refeeding, 24 h pooled faecal water samples were also analyzed. Urinary metabolites upregulated by starvation included 2-butanamidoacetate, 3-hydroxyisovalerate, ketoleucine, methylmalonate, p-cresyl glucuronide, p-cresyl sulfate, phenylacetylglycine, pseudouridine, creatinine, taurine, and N-acetyl glycoprotein, which were related to renal and skeletal muscle function, β-oxidation, turnover of proteins and RNA, and host–microbial interactions. Food-derived metabolites, including gut microbial cometabolites, and tricarboxylic acid cycle intermediates were upregulated under refed and recovered conditions, which characterized anabolic urinary metabotypes. The upregulation of creatine and pantothenate indicated an absorptive state after refeeding. Fecal short chain fatty acids, 3-(3-hydroxyphenyl)propionate, lactate, and acetoin provided additional information about the combinatorial metabolism between the host and gut microbiota. This investigation contributes to allow a deeper understanding of physiological responses associated with starvation and refeeding.
AU  - Serrano-Contreras,JI
AU  - Garcia-Perez,I
AU  - Melendez-Camargo,ME
AU  - Gerardo,Zepeda L
DO  - 10.1021/acs.jproteome.6b00433
EP  - 3254
PY  - 2016///
SN  - 1535-3893
SP  - 3241
TI  - NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/acs.jproteome.6b00433
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000382713300029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/49441
VL  - 15
ER  -
Download