Imperial College London
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ProfessorIolyKotta-Loizou

Faculty of Natural Sciences, Department of Life Sciences

Honorary Lecturer
 
 
 
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Contact

 

i.kotta-loizou13

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kotta-Loizou:2019:10.1128/JVI.02106-18,
author = {Kotta-Loizou, I and Hadrien, P and Saunders, K and Coutts, R and Lomonossoff, G},
doi = {10.1128/JVI.02106-18},
journal = {Journal of Virology},
pages = {1--18},
title = {Investigating the biological relevance of in vitro identified putative packaging signals at the 5’ terminus of the satellite tobacco necrosis virus-1 genomic RNA},
url = {http://dx.doi.org/10.1128/JVI.02106-18},
volume = {93},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Satellite tobacco necrosis virus-1 (STNV-1) is a model system for in vitro RNA encapsidation studies (Patel et al., PNAS 2015, 2017), leading to the identification of degenerate packaging signals (PSs) proposed to be involved in the recognition of its genome by the capsid protein (CP). The aim of the present work was to investigate whether these putative PSs can confer selective packaging of STNV-1 RNA in vivo and assess the prospects of using decoy RNAs in antiviral therapy. We have developed an in planta packaging assay based on the transient expression of STNV-1 CP and assessed the ability of the resulting VLPs to encapsidate mutant STNV-1 RNAs expected to have different encapsidation potential based on in vitro studies. The results revealed that >90% of the encapsidated RNAs are host-derived, although there is some selectivity of packaging for STNV-1 RNA and certain host RNAs. Comparison of the packaging efficiency of mutant STNV-1 RNAs showed that they are encapsidated mainly according to their abundance within the cells, rather than the presence or absence of the putative PSs previously identified from in vitro studies. By contrast, subsequent infection experiments demonstrated that host RNAs represent only <1% of virion content. Although selective encapsidation of certain host RNAs was noted, no direct correlation could be made between this preference and the presence of potential PSs in the host RNA sequences. Overall the data illustrate the differences in RNA packaging efficiency identified through in vitro studies are insufficient to explain the specific packaging of STNV-1 RNA.
AU  - Kotta-Loizou,I
AU  - Hadrien,P
AU  - Saunders,K
AU  - Coutts,R
AU  - Lomonossoff,G
DO  - 10.1128/JVI.02106-18
EP  - 18
PY  - 2019///
SN  - 1098-5514
SP  - 1
TI  - Investigating the biological relevance of in vitro identified putative packaging signals at the 5’ terminus of the satellite tobacco necrosis virus-1 genomic RNA
T2  - Journal of Virology
UR  - http://dx.doi.org/10.1128/JVI.02106-18
UR  - https://jvi.asm.org/content/93/9/e02106-18
UR  - http://hdl.handle.net/10044/1/66416
VL  - 93
ER  -
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