Imperial College London
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ProfessorIolyKotta-Loizou

Faculty of Natural Sciences, Department of Life Sciences

Honorary Lecturer
 
 
 
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Contact

 

i.kotta-loizou13

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Engl:2020:10.1038/s41467-020-16367-6,
author = {Engl, C and Jovanovic, G and Brackston, RD and Kotta-Loizou, I and Buck, M},
doi = {10.1038/s41467-020-16367-6},
journal = {Nature Communications},
title = {The route to transcription initiation determines the mode of transcriptional bursting in E. coli},
url = {http://dx.doi.org/10.1038/s41467-020-16367-6},
volume = {11},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Transcription is fundamentally noisy, leading to significant heterogeneity across bacterial populations. Noise is often attributed to burstiness, but the underlying mechanisms and their dependence on the mode of promotor regulation remain unclear. Here, we measure E. coli single cell mRNA levels for two stress responses that depend on bacterial sigma factors with different mode of transcription initiation (σ70 and σ54). By fitting a stochastic model to the observed mRNA distributions, we show that the transition from low to high expression of the σ70-controlled stress response is regulated via the burst size, while that of the σ54-controlled stress response is regulated via the burst frequency. Therefore, transcription initiation involving σ54 differs from other bacterial systems, and yields bursting kinetics characteristic of eukaryotic systems.
AU  - Engl,C
AU  - Jovanovic,G
AU  - Brackston,RD
AU  - Kotta-Loizou,I
AU  - Buck,M
DO  - 10.1038/s41467-020-16367-6
PY  - 2020///
SN  - 2041-1723
TI  - The route to transcription initiation determines the mode of transcriptional bursting in E. coli
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-16367-6
UR  - http://hdl.handle.net/10044/1/79803
VL  - 11
ER  -
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