Imperial College London
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ProfessorIolyKotta-Loizou

Faculty of Natural Sciences, Department of Life Sciences

Honorary Lecturer
 
 
 
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i.kotta-loizou13

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vassilaki:2020:10.3390/ijms21186974,
author = {Vassilaki, N and Frakolaki, E and Kalliampakou, KI and Sakellariou, P and Kotta-Loizou, I and Bartenschlager, R and Mavromara, P},
doi = {10.3390/ijms21186974},
journal = {International Journal of Molecular Sciences},
title = {A novel cis-acting RNA structural element embedded in the core coding region of the hepatitis C virus genome directs internal translation initiation of the overlapping core+1 ORF},
url = {http://dx.doi.org/10.3390/ijms21186974},
volume = {21},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Hepatitis C virus (HCV) genome translation is initiated via an internal ribosome entry site (IRES) embedded in the 5′-untranslated region (5′UTR). We have earlier shown that the conserved RNA stem-loops (SL) SL47 and SL87 of the HCV core-encoding region are important for viral genome translation in cell culture and in vivo. Moreover, we have reported that an open reading frame overlapping the core gene in the +1 frame (core+1 ORF) encodes alternative translation products, including a protein initiated at the internal AUG codons 85/87 of this frame (nt 597–599 and 603–605), downstream of SL87, which is designated core+1/Short (core+1/S). Here, we provide evidence for SL47 and SL87 possessing a novel cis-acting element that directs the internal translation initiation of core+1/S. Firstly, using a bicistronic dual luciferase reporter system and RNA-transfection experiments, we found that nucleotides 344–596 of the HCV genotype-1a and -2a genomes support translation initiation at the core+1 frame AUG codons 85/87, when present in the sense but not the opposite orientation. Secondly, site-directed mutagenesis combined with an analysis of ribosome–HCV RNA association elucidated that SL47 and SL87 are essential for this alternative translation mechanism. Finally, experiments using cells transfected with JFH1 replicons or infected with virus-like particles showed that core+1/S expression is independent from the 5′UTR IRES and does not utilize the polyprotein initiation codon, but it requires intact SL47 and SL87 structures. Thus, SL47 and SL87, apart from their role in viral polyprotein translation, are necessary elements for mediating the internal translation initiation of the alternative core+1/S ORF.
AU  - Vassilaki,N
AU  - Frakolaki,E
AU  - Kalliampakou,KI
AU  - Sakellariou,P
AU  - Kotta-Loizou,I
AU  - Bartenschlager,R
AU  - Mavromara,P
DO  - 10.3390/ijms21186974
PY  - 2020///
SN  - 1422-0067
TI  - A novel cis-acting RNA structural element embedded in the core coding region of the hepatitis C virus genome directs internal translation initiation of the overlapping core+1 ORF
T2  - International Journal of Molecular Sciences
UR  - http://dx.doi.org/10.3390/ijms21186974
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000580245000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.mdpi.com/1422-0067/21/18/6974
UR  - http://hdl.handle.net/10044/1/108839
VL  - 21
ER  -
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