Imperial College London
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ProfessorIolyKotta-Loizou

Faculty of Natural Sciences, Department of Life Sciences

Honorary Lecturer
 
 
 
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Contact

 

i.kotta-loizou13

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kotta-Loizou:2022:10.1016/j.isci.2022.105425,
author = {Kotta-Loizou, I and Giuliano, MG and Jovanovic, M and Schaefer, J and Ye, F and Zhang, N and Irakleidi, DA and Liu, X and Zhang, X and Buck, M and Engl, C},
doi = {10.1016/j.isci.2022.105425},
journal = {iScience},
title = {The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain},
url = {http://dx.doi.org/10.1016/j.isci.2022.105425},
volume = {25},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - CRISPR-associated Rossmann fold (CARF) domain signaling underpins modulation of CRISPR-Cas nucleases; however, the RtcR CARF domain controls expression of two conserved RNA repair enzymes, cyclase RtcA and ligase RtcB. Here, we demonstrate that RtcAB are required for RtcR-dependent transcription activation and directly bind to RtcR CARF. RtcAB catalytic activity is not required for complex formation with CARF, but is essential yet not sufficient for RtcRAB-dependent transcription activation, implying the need for an additional RNA repair-dependent activating signal. This signal differs from oligoadenylates, a known ligand of CARF domains, and instead appears to originate from the translation apparatus: RtcB repairs a tmRNA that rescues stalled ribosomes and increases translation elongation speed. Taken together, our data provide evidence for an expanded range for CARF domain signaling, including the first evidence of its control via in trans protein-protein interactions, and a feed-forward mechanism to regulate RNA repair required for a functioning translation apparatus.
AU  - Kotta-Loizou,I
AU  - Giuliano,MG
AU  - Jovanovic,M
AU  - Schaefer,J
AU  - Ye,F
AU  - Zhang,N
AU  - Irakleidi,DA
AU  - Liu,X
AU  - Zhang,X
AU  - Buck,M
AU  - Engl,C
DO  - 10.1016/j.isci.2022.105425
PY  - 2022///
SN  - 2589-0042
TI  - The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain
T2  - iScience
UR  - http://dx.doi.org/10.1016/j.isci.2022.105425
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36388977
UR  - http://hdl.handle.net/10044/1/101051
VL  - 25
ER  -
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