Imperial College London
Alternate

Professor Irene Miguel-Aliaga

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Genetics and Physiology
 
 
 
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Contact

 

+44 (0)20 3383 3907i.miguel-aliaga Website

 
 
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Location

 

232ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Messal:2021:10.1038/s41596-020-00414-z,
author = {Messal, HA and Almagro, J and Zaw, Thin M and Tedeschi, A and Ciccarelli, A and Blackie, L and Anderson, KI and Miguel-Aliaga, I and van, Rheenen J and Behrens, A},
doi = {10.1038/s41596-020-00414-z},
journal = {Nature Protocols},
pages = {239--262},
title = {Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH},
url = {http://dx.doi.org/10.1038/s41596-020-00414-z},
volume = {16},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Advances in light-sheet and confocal microscopy now allow imaging of cleared large biological tissue samples and enable the 3D appreciation of cell and protein localization in their native organ environment. However, the sample preparations for such imaging are often onerous, and their capability for antigen detection is limited. Here, we describe FLASH (fast light-microscopic analysis of antibody-stained whole organs), a simple, rapid, fully customizable technique for molecular phenotyping of intact tissue volumes. FLASH utilizes non-degradative epitope recovery and membrane solubilization to enable the detection of a multitude of membranous, cytoplasmic and nuclear antigens in whole mouse organs and embryos, human biopsies, organoids and Drosophila. Retrieval and immunolabeling of epithelial markers, an obstacle for previous clearing techniques, can be achieved with FLASH. Upon volumetric imaging, FLASH-processed samples preserve their architecture and integrity and can be paraffin-embedded for subsequent histopathological analysis. The technique can be performed by scientists trained in light microscopy and yields results in <1 week.
AU  - Messal,HA
AU  - Almagro,J
AU  - Zaw,Thin M
AU  - Tedeschi,A
AU  - Ciccarelli,A
AU  - Blackie,L
AU  - Anderson,KI
AU  - Miguel-Aliaga,I
AU  - van,Rheenen J
AU  - Behrens,A
DO  - 10.1038/s41596-020-00414-z
EP  - 262
PY  - 2021///
SN  - 1750-2799
SP  - 239
TI  - Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH
T2  - Nature Protocols
UR  - http://dx.doi.org/10.1038/s41596-020-00414-z
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000593453000004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41596-020-00414-z
UR  - http://hdl.handle.net/10044/1/88849
VL  - 16
ER  -
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