Imperial College London
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DrIngridMuller

Faculty of Medicine, Department of Infectious Disease

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120Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Adem:2016:10.1371/journal.pntd.0004468,
author = {Adem, E and Tajebe, F and Getahun, M and Kiflie, A and Diro, E and Hailu, A and Shkedy, Z and Mengesha, B and Mulaw, T and Atnafu, S and Deressa, T and Mathewos, B and Abate, E and Modolell, M and Munder, M and Müller, I and Takele, Y and Kropf, P},
doi = {10.1371/journal.pntd.0004468},
journal = {PLOS Neglected Tropical Diseases},
pages = {e0004468--e0004468},
title = {Successful treatment of human visceral leishmaniasis restores antigen-specific IFN-γ, but not IL-10 production},
url = {http://dx.doi.org/10.1371/journal.pntd.0004468},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-γ (IFN-γ). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to produce IFN-γ. Here we tested the hypothesis that a population of low-density granulocytes (LDG) might contribute to T cell responses hyporesponsiveness via the release of arginase. Our results show that this population is affected by the anticoagulant used to collect blood: the frequency of LDGs is significantly lower when the blood is collected with heparin as compared to EDTA; however, the anticoagulant does not impact on the levels of arginase released. Next, we assessed the capacity of whole blood cells from patients with active VL to produce IFN-γ and IL-10 in response to antigen-specific and polyclonal activation. Our results show that whole blood cells produce low or levels below detection limit of IFN-γ and IL-10, however, after successful treatment of VL patients, these cells gradually regain their capacity to produce IFN-γ, but not IL-10, in response to activation. These results suggest that in contrast to VL patients from Bihar, India, whole blood cells from VL patients from Gondar, Ethiopia, have lost their ability to produce IFN-γ during active VL and that active disease is not associated with sustained levels of IL-10 production following stimulation.
AU  - Adem,E
AU  - Tajebe,F
AU  - Getahun,M
AU  - Kiflie,A
AU  - Diro,E
AU  - Hailu,A
AU  - Shkedy,Z
AU  - Mengesha,B
AU  - Mulaw,T
AU  - Atnafu,S
AU  - Deressa,T
AU  - Mathewos,B
AU  - Abate,E
AU  - Modolell,M
AU  - Munder,M
AU  - Müller,I
AU  - Takele,Y
AU  - Kropf,P
DO  - 10.1371/journal.pntd.0004468
EP  - 0004468
PY  - 2016///
SN  - 1935-2735
SP  - 0004468
TI  - Successful treatment of human visceral leishmaniasis restores antigen-specific IFN-γ, but not IL-10 production
T2  - PLOS Neglected Tropical Diseases
UR  - http://dx.doi.org/10.1371/journal.pntd.0004468
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26962865
UR  - http://hdl.handle.net/10044/1/30957
VL  - 10
ER  -
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