Imperial College London
Alternate

DrIstvanNagy

Faculty of Medicine, Department of Surgery & Cancer

Reader in Nociceptive Mechanisms
 
 
 
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Contact

 

+44 (0)20 3315 8897i.nagy Website

 
 
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Assistant

 

Miss Steffi Klier +44 (0)20 3315 8816

 
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Location

 

G345Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nagy:2018:10.3389/fnins.2018.00732,
author = {Nagy, I and Lazar, BA and Jancso, G and Palvolgyi, L and Dobos, I and Santha, P},
doi = {10.3389/fnins.2018.00732},
journal = {Frontiers in Neuroscience},
title = {Insulin confers differing effects on neurite outgrowth in separate populations of cultured dorsal root ganglion neurons: the role of the insulin receptor},
url = {http://dx.doi.org/10.3389/fnins.2018.00732},
volume = {12},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Apart from its pivotal role in the regulation of carbohydrate metabolism, insulin exerts important neurotrophic and neuromodulator effects on dorsal root ganglion (DRG) neurons. The neurite outgrowth-promoting effect is one of the salient features of insulin’s action on cultured DRG neurons. Although it has been established that a significant population of DRG neurons express the insulin receptor (InsR), the significance of InsR expression and the chemical phenotype of DRG neurons in relation to the neurite outgrowth-promoting effect of insulin has not been studied. Therefore, in this study by using immunohistochemical and quantitative stereological methods we evaluated the effect of insulin on neurite outgrowth of DRG neurons of different chemical phenotypes which express or lack the InsR. Insulin, at a concentration of 10 nM, significantly increased total neurite length, the length of the longest neurite and the number of branch points of cultured DRG neurons as compared to neurons cultured in control medium or in the presence of 1 μM insulin. In both the control and the insulin exposed cultures, ∼43% of neurons displayed InsR-immunoreactivity. The proportions of transient receptor potential vanilloid type 1 receptor (TRPV1)-immunoreactive (IR), calcitonin gene-related peptide (CGRP)-IR and Bandeiraea simplicifolia isolectin B4 (IB4)-binding neurons amounted to ∼61%, ∼57%, and ∼31% of DRG neurons IR for the InsR. Of the IB4-positive population only neurons expressing the InsR were responsive to insulin. In contrast, TRPV1-IR nociceptive and CGRP-IR peptidergic neurons showed increased tendency for neurite outgrowth which was further enhanced by insulin. However, the responsiveness of DRG neurons expressing the InsR was superior to populations of DRG neurons which lack this receptor. The findings also revealed that besides the expression of the InsR, inherent properties of peptidergic, but not non-peptidergic nociceptive neurons may also si
AU  - Nagy,I
AU  - Lazar,BA
AU  - Jancso,G
AU  - Palvolgyi,L
AU  - Dobos,I
AU  - Santha,P
DO  - 10.3389/fnins.2018.00732
PY  - 2018///
SN  - 1662-4548
TI  - Insulin confers differing effects on neurite outgrowth in separate populations of cultured dorsal root ganglion neurons: the role of the insulin receptor
T2  - Frontiers in Neuroscience
UR  - http://dx.doi.org/10.3389/fnins.2018.00732
UR  - http://hdl.handle.net/10044/1/65030
VL  - 12
ER  -
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