Imperial College London
Alternate

DrIstvanNagy

Faculty of Medicine, Department of Surgery & Cancer

Reader in Nociceptive Mechanisms
 
 
 
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Contact

 

+44 (0)20 3315 8897i.nagy Website

 
 
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Assistant

 

Miss Steffi Klier +44 (0)20 3315 8816

 
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Location

 

G345Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nagy:2021:10.3390/ijms22052297,
author = {Nagy, I and Varga, A and Meszar, Z and Sivado, M and Bacskai, T and Vegh, B and Kokai, E and Szucs, P},
doi = {10.3390/ijms22052297},
journal = {International Journal of Molecular Sciences},
pages = {1--28},
title = {Spinal excitatory dynorphinergic interneurons contribute to burn injury-induced nociception mediated by phosphorylated histone 3 at serine 10 in rodents},
url = {http://dx.doi.org/10.3390/ijms22052297},
volume = {22},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The phosphorylation of serine 10 in histone 3 (p-S10H3) has recently been demonstrated to participate in spinal nociceptive processing. However, superficial dorsal horn (SDH) neurons involved in p-S10H3-mediated nociception have not been fully characterized. In the present work, we combined immunohistochemistry, in situ hybridization with the retrograde labeling of projection neurons to reveal the subset of dorsal horn neurons presenting an elevated level of p-S10H3 in response to noxious heat (60 °C), causing burn injury. Projection neurons only represented a small percentage (5%) of p-S10H3-positive cells, while the greater part of them belonged to excitatory SDH interneurons. The combined immunolabeling of p-S10H3 with markers of already established interneuronal classes of the SDH revealed that the largest subset of neurons with burn injury-induced p-S10H3 expression was dynorphin immunopositive in mice. Furthermore, the majority of p-S10H3-expressing dynorphinergic neurons proved to be excitatory, as they lacked Pax-2 and showed Lmx1b-immunopositivity. Thus, we showed that neurochemically heterogeneous SDH neurons exhibit the upregulation of p-S10H3 shortly after noxious heat-induced burn injury and consequential tissue damage, and that a dedicated subset of excitatory dynorphinergic neurons is likely a key player in the development of central sensitization via the p-S10H3 mediated pathway.
AU  - Nagy,I
AU  - Varga,A
AU  - Meszar,Z
AU  - Sivado,M
AU  - Bacskai,T
AU  - Vegh,B
AU  - Kokai,E
AU  - Szucs,P
DO  - 10.3390/ijms22052297
EP  - 28
PY  - 2021///
SN  - 1422-0067
SP  - 1
TI  - Spinal excitatory dynorphinergic interneurons contribute to burn injury-induced nociception mediated by phosphorylated histone 3 at serine 10 in rodents
T2  - International Journal of Molecular Sciences
UR  - http://dx.doi.org/10.3390/ijms22052297
UR  - https://www.mdpi.com/1422-0067/22/5/2297
UR  - http://hdl.handle.net/10044/1/88217
VL  - 22
ER  -
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