Imperial College London
Alternate

DrIstvanNagy

Faculty of Medicine, Department of Surgery & Cancer

Reader in Nociceptive Mechanisms
 
 
 
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Contact

 

+44 (0)20 3315 8897i.nagy Website

 
 
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Assistant

 

Miss Steffi Klier +44 (0)20 3315 8816

 
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Location

 

G345Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sousa:2021:10.3390/ijms22126380,
author = {Sousa, Valente J and Alavi, J and Barde, S and Zarban, A and Kodji, X and That, D and Argunham, F and Barrett, B and Nagy, I and Brain, SD},
doi = {10.3390/ijms22126380},
journal = {International Journal of Molecular Sciences},
title = {(-)-Englerin-A has analgesic and anti-inflammatory effects independent of TRPC4 and 5},
url = {http://dx.doi.org/10.3390/ijms22126380},
volume = {22},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Recently, we found that the deletion of TRPC5 leads to increased inflammation and pain-related behaviour in two animal models of arthritis. (-)-Englerin A (EA), an extract from the East African plant Phyllanthus engleri has been identified as a TRPC4/5 agonist. Here, we studied whether or not EA has any anti-inflammatory and analgesic properties via TRPC4/5 in the carrageenan model of inflammation. We found that EA treatment in CD1 mice inhibited thermal hyperalgesia and mechanical allodynia in a dose-dependent manner. Furthermore, EA significantly reduced the volume of carrageenan-induced paw oedema and the mass of the treated paws. Additionally, in dorsal root ganglion (DRG) neurons cultured from WT 129S1/SvIm mice, EA induced a dose-dependent cobalt uptake that was surprisingly preserved in cultured DRG neurons from 129S1/SvIm TRPC5 KO mice. Likewise, EA-induced anti-inflammatory and analgesic effects were preserved in the carrageenan model in animals lacking TRPC5 expression or in mice treated with TRPC4/5 antagonist ML204.This study demonstrates that while EA activates a sub-population of DRG neurons, it induces a novel TRPC4/5-independent analgesic and anti-inflammatory effect in vivo. Future studies are needed to elucidate the molecular and cellular mechanisms underlying EA’s anti-inflammatory and analgesic effects.
AU  - Sousa,Valente J
AU  - Alavi,J
AU  - Barde,S
AU  - Zarban,A
AU  - Kodji,X
AU  - That,D
AU  - Argunham,F
AU  - Barrett,B
AU  - Nagy,I
AU  - Brain,SD
DO  - 10.3390/ijms22126380
PY  - 2021///
SN  - 1422-0067
TI  - (-)-Englerin-A has analgesic and anti-inflammatory effects independent of TRPC4 and 5
T2  - International Journal of Molecular Sciences
UR  - http://dx.doi.org/10.3390/ijms22126380
UR  - http://hdl.handle.net/10044/1/90466
VL  - 22
ER  -
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