Imperial College London
Alternate

DrIsabelleSalles

Faculty of Medicine, Department of Immunology and Inflammation

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 2298i.salles

 
 
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Location

 

5S5Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Salles-Crawley:2014:10.1182/blood-2013-10-534024,
author = {Salles-Crawley, II and Monkman, JH and Ahnstroem, J and Lane, DA and Crawley, JTB},
doi = {10.1182/blood-2013-10-534024},
journal = {Blood},
pages = {2873--2881},
title = {Vessel wall BAMBI contributes to hemostasis and thrombus stability.},
url = {http://dx.doi.org/10.1182/blood-2013-10-534024},
volume = {123},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein related to the transforming growth factor-β superfamily, and is highly expressed in platelets and endothelial cells. We previously demonstrated its positive role in thrombus formation using a zebrafish thrombosis model. In the present study, we used Bambi-deficient mice and radiation chimeras to evaluate the function of this receptor in the regulation of both hemostasis and thrombosis. We show that Bambi−/− and Bambi+/− mice exhibit mildly prolonged bleeding times compared with Bambi+/+ littermates. In addition, using 2 in vivo thrombosis models in mesenterium or cremaster muscle arterioles, we demonstrate that Bambi-deficient mice form unstable thrombi compared with Bambi+/+ mice. No defects in thrombin generation in Bambi−/− mouse plasma could be detected ex vivo. Moreover, the absence of BAMBI had no effect on platelet counts, platelet activation, aggregation, or platelet procoagulant function. Similar to Bambi−/− mice, Bambi−/− transplanted with Bambi+/+ bone marrow formed unstable thrombi in the laser-induced thrombosis model that receded more rapidly than thrombi that formed in Bambi+/+ mice receiving Bambi−/− bone marrow transplants. Taken together, these results provide strong evidence for an important role of endothelium rather than platelet BAMBI as a positive regulator of both thrombus formation and stability.
AU  - Salles-Crawley,II
AU  - Monkman,JH
AU  - Ahnstroem,J
AU  - Lane,DA
AU  - Crawley,JTB
DO  - 10.1182/blood-2013-10-534024
EP  - 2881
PY  - 2014///
SN  - 0006-4971
SP  - 2873
TI  - Vessel wall BAMBI contributes to hemostasis and thrombus stability.
T2  - Blood
UR  - http://dx.doi.org/10.1182/blood-2013-10-534024
UR  - http://hdl.handle.net/10044/1/61073
VL  - 123
ER  -
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