Professor Irene Roberts

Roberts I, McCloy M, 2001, Obstetrics, fetal and paediatric, Pages: 87-107, ISBN: 9780632051144

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Carapagnoli C, Fisk NM, Kumar S, Bellantuono L, Bennett PR, Roberts IAGet al., 2000, Identification of mesenchymal stem cells in human first trimester fetal blood, liver and bone marrow, Blood, Vol: 96, ISSN: 0006-4971

Human mesenchymal stem cells (MSC) can be isolated from adult bone marrow and either extensively expanded without differentiation or induced to differentiate into multiple mesenchymal lineages. The aim of this study was to investigate the characteristics of MSC during the first trimester of human life. First trimester fetal blood (n=15; median gest age: 10: wks), fetal liver (n=2,10° and 14° wks) and fetal bone marrow (n=2, 11 2 and 14 wks) samples were collected and nucleated cells cultured at 10 5/ml in DMEM with 10% FBS.Single fibroblastic-like colonies were stained and scored: mean number (± SEM) of colonies: 8.2 ±0.6, 11.3± 2.0 and 12.6± 3.6/10 nucleated cells from fetal blood, liver and bone marrow , respectively. Fibroblast-like cell colonies from first trimester fetal blood (n=3; median gest age: 93 wks), liver (n=l; 14 wks) and bone marrow (n=l, II2 wks) were plucked after 2 passages in culture.Single colony- derived cells could be expanded in vitro for at least 20 passages. Fetal blood, liver and bone marrow derived single colonies showed mean cumulative population doublings of 50.3 ±4.5, 52.2 ±5.3 and 49.5 ±3.5, respectively. In their undifferentiated state, clonal adherent cells from fetal blood, liver and bone marrow did not express CD45, CD34, CD14, CD68, vWF, HLA-DR antigens while they produced prolyl-4-hydroxylase, a-smooth muscle actin, fibronectin, laminin, vimentin and reacted positively with mesenchymal cell specific antibodies (SH2, SH3, SH4). When individual fetal blood, liver and bone marrow adherent cell colonies were cultured in hydrocortisone, isobutylmethylxanthine and indomethacin for 2 weeks the cells underwent adipogenic differentiation, displaying large vacuoles which were oil red O positive; after 2 weeks of culture in the presence of osteogenic medium (dexamethasone, ascorbic acid, β-glycerophosphate) calcium deposition was detected by silver nitrate and Alizarin red stain

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Karadimitris A, Notaro R, Koehne G, Roberts IAG, Luzzatto Let al., 2000, PNH cells are as sensitive to T-cell-mediated lysis as their normal counterparts: implications for the pathogenesis of paroxysmal nocturnal haemoglobinuria, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 111, Pages: 1158-1163, ISSN: 0007-1048

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• Citations: 29

Campagnoli C, Fisk NM, Kumar S, Bellantuono I, Bennett PR, Roberts IAGet al., 2000, Identification of mesenchymal stem cells in human first trimester fetal blood, liver and bone marrow., BLOOD, Vol: 96, Pages: 279A-279A, ISSN: 0006-4971

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• Citations: 1

Karadimitris A, Manavalan JS, Thaler HT, Notaro R, Araten DJ, Nafa K, Roberts IAG, Weksler ME, Luzzatto Let al., 2000, Abnormal T-cell repertoire is consistent with immune process underlying the pathogenesis of paroxysmal nocturnal hemoglobinuria, BLOOD, Vol: 96, Pages: 2613-2620, ISSN: 0006-4971

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• Citations: 97

Choolani M, O'Donnell H, Campagnoli C, Kumar S, Roberts I, Bennett P, Fisk NMet al., 2000, Simultaneous visualization of fetal cell marker and chromosomal FISH for prenatal diagnosis using fetal erythroblasts in maternal blood: Development of a novel technique., AMERICAN JOURNAL OF HUMAN GENETICS, Vol: 67, Pages: 422-422, ISSN: 0002-9297

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• Citations: 2

Campagnoli C, Fisk N, Overton T, Bennett P, Watts T, Roberts Iet al., 2000, Circulating hematopoietic progenitor cells in first trimester fetal blood, BLOOD, Vol: 95, Pages: 1967-1972, ISSN: 0006-4971

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• Citations: 58

Marley SB, Lewis JL, Davidson RJ, Roberts IAG, Dokal I, Goldman JM, Gordon MYet al., 1999, Evidence for a continuous decline in haemopoietic cell function from birth: application to evaluating bone marrow failure in children, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 106, Pages: 162-166, ISSN: 0007-1048

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• Citations: 67

Roberts IAG, Murray NA, 1999, Management of thrombocytopenia in neonates, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 105, Pages: 864-870, ISSN: 0007-1048

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• Citations: 25

Duggan PJ, Maalouf EF, Watts T, Sullivan MH, Counsell SJ, Fletcher AM, Rutherford MA, Al-Nakib LA, Ajayi-Obe M, Battin M, Cowan F, Cox F, Roberts I, Edwards ADet al., 1999, Fetal T-cell activation and inflammation are related to prenatal brain injury, PEDIATRIC RESEARCH, Vol: 45, Pages: 261A-261A, ISSN: 0031-3998

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Campagnoli C, Fisk N, Overton T, Bennett P, Roberts Iet al., 1999, Circulating haemopoietic progenitor cells in first trimester fetal blood., BRITISH JOURNAL OF HAEMATOLOGY, Vol: 105, Pages: 65-65, ISSN: 0007-1048

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Chase A, Reiter A, Burci L, Cazzaniga G, Biondi A, Pickard J, Roberts IAG, Goldman JM, Cross NCPet al., 1999, Fusion of ETV6 to the caudal-related homeobox gene CDX2 in acute myeloid leukemia with the t(12;13)(p13;q12), BLOOD, Vol: 93, Pages: 1025-1031, ISSN: 0006-4971

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• Citations: 88

Shlebak AA, Marley SB, Roberts IAG, Davidson RJ, Goldman JM, Gordon MYet al., 1999, Optimal timing for processing and cryopreservation of umbilical cord haematopoietic stem cells for clinical transplantation, BONE MARROW TRANSPLANTATION, Vol: 23, Pages: 131-136, ISSN: 0268-3369

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• Citations: 27

Shlebak AA, Roberts IAG, Stevens TA, Syzdlo RM, Goldman JM, Gordon MYet al., 1998, The impact of antenatal and perinatal variables on cord blood haemopoietic stem/progenitor cell yield available for transplantation, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 103, Pages: 1167-1171, ISSN: 0007-1048

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• Citations: 54

Chase A, Reiter A, Burci L, Cazzaniga G, Biondi A, Pickard J, Roberts IAG, Goldman JM, Cross NCPet al., 1998, ETV6 is fused to the homeobox gene CDX2 in a patient with acute myeloid leukemia and a t(12;13)(p13;q12)., BLOOD, Vol: 92, Pages: 70A-70A, ISSN: 0006-4971

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• Citations: 1

Campagnoli C, Fisk N, Bennett P, Overton T, Roberts Iet al., 1998, Circulating multipotent haemopoietic progenitors in first trimester fetal blood., BLOOD, Vol: 92, Pages: 142B-142B, ISSN: 0006-4971

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Amrolia PJ, Kaeda J, Vulliamy T, Roper D, Dokal I, Roberts IAGet al., 1998, Analysis of chimerism in children with haemoglobinopathies undergoing stem cell transplantation., Publisher: BLACKWELL SCIENCE LTD, Pages: 66-66, ISSN: 0007-1048

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• Citations: 1

Shlebak AA, Marley SB, Roberts IAG, Goldman JM, Gordon MYet al., 1998, Variables affecting umbilical cord blood progenitor stem cell yield for clinical transplantation., BRITISH JOURNAL OF HAEMATOLOGY, Vol: 101, Pages: 96-96, ISSN: 0007-1048

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Vaughan JI, Manning M, Warwick RM, Letsky EA, Murray NA, Roberts IAGet al., 1998, Inhibition of erythroid progenitor cells by anti-Kell antibodies in fetal alloimmune anemia, NEW ENGLAND JOURNAL OF MEDICINE, Vol: 338, Pages: 798-803, ISSN: 0028-4793

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• Citations: 172

Roberts IAG, Niethammer D, van Biezen A, Apperley JFet al., 1998, Outcome of transplant for children with adult type CML (ACTML)., BONE MARROW TRANSPLANTATION, Vol: 21, Pages: S85-S85, ISSN: 0268-3369

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Dufour C, Maher J, Murray N, Manning M, Dokal I, Luzzatto L, Roberts IAGet al., 1998, An unusual case of familial aplastic anaemia:: <i>in vitro</i> and <i>in vivo</i> evidence for a multipotent progenitor responsive to G-CSF, EUROPEAN JOURNAL OF HAEMATOLOGY, Vol: 60, Pages: 209-212, ISSN: 0902-4441

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O'Donnell J, Goldman JM, Wagner K, Ehinger G, Martin N, Leahy M, Kariuki N, Roberts Iet al., 1998, Donor-derived <i>Plasmodium vivax</i> infection following volunteer unrelated bone marrow transplantation, BONE MARROW TRANSPLANTATION, Vol: 21, Pages: 313-314, ISSN: 0268-3369

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• Citations: 20

Murray NA, Watts TL, Roberts IAG, 1998, Endogenous thrombopoietin levels and effect of recombinant human thrombopoietin on megakaryocyte precursors in term and preterm babies, PEDIATRIC RESEARCH, Vol: 43, Pages: 148-151, ISSN: 0031-3998

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• Citations: 80

ODonnell J, Roberts I, DelaFuente J, Daly P, New H, Dokal Iet al., 1997, Successful second bone marrow transplant for Fanconi's anaemia following escalation of conditioning, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 98, Pages: 772-774, ISSN: 0007-1048

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• Citations: 4

Roberts I, 1997, Current status of allogeneic transplantation for haemoglobinopathies, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 98, Pages: 1-7, ISSN: 0007-1048

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• Citations: 15

Chen FE, Owen I, Savage D, Roberts I, Apperley J, Goldman JM, Laffan Met al., 1997, Late onset haemolysis and red cell autoimmunisation after allogeneic bone marrow transplant, BONE MARROW TRANSPLANTATION, Vol: 19, Pages: 491-495, ISSN: 0268-3369

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• Citations: 78

LeDeist F, Emile JF, RieuxLaucat F, Benkerrou M, Roberts I, Brousse N, Fischer Aet al., 1996, Clinical, immunological, and pathological consequences of Fas-deficient conditions, LANCET, Vol: 348, Pages: 719-723, ISSN: 0140-6736

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• Citations: 157

Patel J, Pryds O, Roberts I, Harris D, Edwards ADet al., 1996, Limited role for nitric oxide in mediating cerebrovascular control of newborn piglets, ARCHIVES OF DISEASE IN CHILDHOOD-FETAL AND NEONATAL EDITION, Vol: 75, Pages: F82-F86, ISSN: 1359-2998

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• Citations: 6

Dokal IS, Roberts IAG, 1996, Bone marrow transplantation for Fanconi's anaemia: Conditioning with reduced doses of cyclophosphamide without radiation, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 94, Pages: 423-423, ISSN: 0007-1048

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• Citations: 9

Dokal IS, Roberts IA, 1996, Bone marrow transplantation for Fanconi's anaemia: conditioning with reduced doses of cyclophosphamide without radiation., Br J Haematol, Vol: 94, ISSN: 0007-1048

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