Imperial College London
Alternate

Professor Irene Roberts

Faculty of Medicine, Department of Immunology and Inflammation

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 3313 2163irene.roberts

 
 
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Assistant

 

Miss Karen Linfield +44 (0)20 3313 3238

 
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Location

 

4S10BHammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pitcher:2014,
author = {Pitcher, DS and De, Mattos-Shipley K and Wang, Z and Tzortzis, K and Goudevenou, K and Flynn, H and Bohn, G and Rahemtulla, A and Roberts, I and Snijders, AP and Karadimitris, A and Kleijnen, MF},
journal = {Biochimica et Biophysica Acta - Proteins and Proteomics},
pages = {2222--2228},
title = {Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE)},
url = {http://hdl.handle.net/10044/1/23208},
volume = {1844},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We report that subunits of human nuclear proteasomes carry a previously unrecognised, constitutive posttranslational modification. Subunits with this modification are not visualised by SDS-PAGE, which is used in almost all denaturing protein gel electrophoresis. In contrast, CTAB-PAGE readily visualises such modified subunits. Thus, under most experimental conditions, with identical samples, SDS-PAGE yielded gel electrophoresis patterns for subunits of nuclear proteasomes which were misleading and strikingly different from those obtained with CTAB-PAGE. Initial analysis indicates a novel modification of a high negative charge with some similarity to polyADP-ribose, possibly explaining compatibility with (positively-charged) CTAB-PAGE but not (negatively-charged) SDS-PAGE and providing a mechanism for how nuclear proteasomes may interact with chromatin, DNA and other nuclear components.
AU  - Pitcher,DS
AU  - De,Mattos-Shipley K
AU  - Wang,Z
AU  - Tzortzis,K
AU  - Goudevenou,K
AU  - Flynn,H
AU  - Bohn,G
AU  - Rahemtulla,A
AU  - Roberts,I
AU  - Snijders,AP
AU  - Karadimitris,A
AU  - Kleijnen,MF
EP  - 2228
PY  - 2014///
SP  - 2222
TI  - Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE)
T2  - Biochimica et Biophysica Acta - Proteins and Proteomics
UR  - http://hdl.handle.net/10044/1/23208
VL  - 1844
ER  -
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