Imperial College London
Dr. Isra Marei

Dr. Isra Marei

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Fellow
 
 
 
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Contact

 

isra.marei11 Website

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ahmetaj-Shala:2021:10.3389/fcell.2021.681347,
author = {Ahmetaj-Shala, B and Marei, I and Kawai, R and Rothery, S and Pericleous, C and Mohamed, N and Gashaw, H and Bokea, K and Samuel, J and Vandenheste, A and Shala, F and Kirkby, N and Mitchell, J},
doi = {10.3389/fcell.2021.681347},
journal = {Frontiers in Cell and Developmental Biology},
pages = {1--6},
title = {Activation and contraction of human ‘vascular’ smooth muscle cells grown from circulating blood progenitors},
url = {http://dx.doi.org/10.3389/fcell.2021.681347},
volume = {9},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Blood outgrowth smooth muscle cells offer the means to study vascular cells without the requirement for surgery providing opportunities for drug discovery, tissue engineering and personalised medicine. However, little is known about these cells which has meant their therapeutic potential remains unexplored. Our objective was to investigate for the first time the ability of blood outgrowth smooth muscle cells and vessel derived smooth muscle cells to sense the thromboxane mimetic U46619 bymeasuring intracellular calcium elevation and contraction. U46619 (10 26 -6M) increased cytosolic calcium in blood outgrowth smooth muscle cells fibroblasts. Increased calcium signal peaked between 10-20 seconds after U46619 in both smoothmuscle cell types. Importantly, U46619 (10-9 to 10-6M) induced concentration-dependent contractions of both blood outgrowth smooth muscle cells and vascular smooth muscle cells but not in fibroblasts. In summary, we show that functional responses of blood outgrowth smooth muscle cells are in line with vascular smooth muscle cells providing critical evidence of their application in biomedical research.
AU  - Ahmetaj-Shala,B
AU  - Marei,I
AU  - Kawai,R
AU  - Rothery,S
AU  - Pericleous,C
AU  - Mohamed,N
AU  - Gashaw,H
AU  - Bokea,K
AU  - Samuel,J
AU  - Vandenheste,A
AU  - Shala,F
AU  - Kirkby,N
AU  - Mitchell,J
DO  - 10.3389/fcell.2021.681347
EP  - 6
PY  - 2021///
SN  - 2296-634X
SP  - 1
TI  - Activation and contraction of human ‘vascular’ smooth muscle cells grown from circulating blood progenitors
T2  - Frontiers in Cell and Developmental Biology
UR  - http://dx.doi.org/10.3389/fcell.2021.681347
UR  - https://www.frontiersin.org/articles/10.3389/fcell.2021.681347/full
UR  - http://hdl.handle.net/10044/1/90918
VL  - 9
ER  -
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