Imperial College London
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DrJorgeBernardino de la Serna

Faculty of Medicine, National Heart & Lung Institute

Senior Lecturer in Inhalation Toxicology and Pharmacology
 
 
 
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Contact

 

+44 (0)20 7594 3277j.bernardino-de-la-serna Website

 
 
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Location

 

CubicleSir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bernabe-Rubio:2021:10.1002/smtd.202000711,
author = {Bernabe-Rubio, M and Bosch-Fortea, M and Garcia, E and Bernardino, de la Serna J and Alonso, MA},
doi = {10.1002/smtd.202000711},
journal = {Small Methods},
title = {Adaptive lipid immiscibility and Membrane remodeling are active functional Determinants of primary ciliogenesis},
url = {http://dx.doi.org/10.1002/smtd.202000711},
volume = {5},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Small Methods published by Wiley-VCH GmbH Lipid liquid–liquid immiscibility and its consequent lateral heterogeneity have been observed under thermodynamic equilibrium in model and native membranes. However, cholesterol-rich membrane domains, sometimes referred to as lipid rafts, are difficult to observe spatiotemporally in live cells. Despite their importance in many biological processes, robust evidence for their existence remains elusive. This is mainly due to the difficulty in simultaneously determining their chemical composition and physicochemical nature, whilst spatiotemporally resolving their nanodomain lifetime and molecular dynamics. In this study, a bespoke method based on super-resolution stimulated emission depletion (STED) microscopy and raster imaging correlation spectroscopy (RICS) is used to overcome this issue. This methodology, laser interleaved confocal RICS and STED-RICS (LICSR), enables simultaneous tracking of lipid lateral packing and dynamics at the nanoscale. Previous work indicated that, in polarized epithelial cells, the midbody remnant licenses primary cilium formation through an unidentified mechanism. LICSR shows that lipid immiscibility and its adaptive collective nanoscale self-assembly are crucial for the midbody remnant to supply condensed membranes to the centrosome for the biogenesis of the ciliary membrane. Hence, this work poses a breakthrough in the field of lipid biology by providing compelling evidence of a functional role for liquid ordered-like membranes in primary ciliogenesis.
AU  - Bernabe-Rubio,M
AU  - Bosch-Fortea,M
AU  - Garcia,E
AU  - Bernardino,de la Serna J
AU  - Alonso,MA
DO  - 10.1002/smtd.202000711
PY  - 2021///
SN  - 2366-9608
TI  - Adaptive lipid immiscibility and Membrane remodeling are active functional Determinants of primary ciliogenesis
T2  - Small Methods
UR  - http://dx.doi.org/10.1002/smtd.202000711
UR  - http://hdl.handle.net/10044/1/85948
VL  - 5
ER  -
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