Imperial College London
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DrJorgeBernardino de la Serna

Faculty of Medicine, National Heart & Lung Institute

Senior Lecturer in Inhalation Toxicology and Pharmacology
 
 
 
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Contact

 

+44 (0)20 7594 3277j.bernardino-de-la-serna Website

 
 
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Location

 

CubicleSir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bernabé-Rubio:2021:10.1016/j.ymeth.2021.06.007,
author = {Bernabé-Rubio, M and Bosch-Fortea, M and Alonso, MA and Bernardino, de la Serna J},
doi = {10.1016/j.ymeth.2021.06.007},
journal = {Methods},
title = {Multi-dimensional and spatiotemporal correlative imaging at the plasma membrane of live cells to determine the continuum nano-to-micro scale lipid adaptation and collective motion.},
url = {http://dx.doi.org/10.1016/j.ymeth.2021.06.007},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The primary cilium is a specialized plasma membrane protrusion with important receptors for signalling pathways. In polarized epithelial cells, the primary cilium assembles after the midbody remnant (MBR) encounters the centrosome at the apical surface. The membrane surrounding the MBR, namely remnant-associated membrane patch (RAMP), once situated next to the centrosome, releases some of its lipid components to form a centrosome-associated membrane patch (CAMP) from which the ciliary membrane stems. The RAMP undergoes a spatiotemporal membrane refinement during the formation of the CAMP, which becomes highly enriched in condensed membranes with low lateral mobility. To better understand this process, we have developed a correlative imaging approach that yields quantitative information about the lipid lateral packing, its mobility and collective assembly at the plasma membrane at different spatial scales over time. Our work paves the way towards a quantitative understanding of the spatiotemporal lipid collective assembly at the plasma membrane as a functional determinant in cell biology and its direct correlation with the membrane physicochemical state. These findings allowed us to gain a deeper insight into the mechanisms behind the biogenesis of the ciliary membrane of polarized epithelial cells.
AU  - Bernabé-Rubio,M
AU  - Bosch-Fortea,M
AU  - Alonso,MA
AU  - Bernardino,de la Serna J
DO  - 10.1016/j.ymeth.2021.06.007
PY  - 2021///
SN  - 1046-2023
TI  - Multi-dimensional and spatiotemporal correlative imaging at the plasma membrane of live cells to determine the continuum nano-to-micro scale lipid adaptation and collective motion.
T2  - Methods
UR  - http://dx.doi.org/10.1016/j.ymeth.2021.06.007
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34126167
UR  - https://www.sciencedirect.com/science/article/pii/S1046202321001596?via%3Dihub
ER  -
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