Imperial College London
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DrJorgeBernardino de la Serna

Faculty of Medicine, National Heart & Lung Institute

Senior Lecturer in Inhalation Toxicology and Pharmacology
 
 
 
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Contact

 

+44 (0)20 7594 3277j.bernardino-de-la-serna Website

 
 
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Location

 

CubicleSir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lorizate:2021:10.1002/smtd.202100430,
author = {Lorizate, M and Terrones, O and NietoGarai, JA and RojoBartolomé, I and Ciceri, D and Morana, O and OlazarIntxausti, J and Arboleya, A and Martin, A and Szynkiewicz, M and CallejaFelipe, M and Bernardino, de la Serna J and Contreras, F},
doi = {10.1002/smtd.202100430},
journal = {Small Methods},
pages = {1--15},
title = {Superresolution microscopy using a bioorthogonalbased cholesterol probe provides unprecedented capabilities for imaging nanoscale lipid heterogeneity in living cells},
url = {http://dx.doi.org/10.1002/smtd.202100430},
volume = {5},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Despite more than 20 years of work since the lipid raft concept was proposed, the existence of these nanostructures remains highly controversial due to the lack of noninvasive methods to investigate their native nanorganization in living unperturbed cells. There is an unmet need for probes for direct imaging of nanoscale membrane dynamics with high spatial and temporal resolution in living cells. In this paper, a bioorthogonal-based cholesterol probe (chol-N3) is developed that, combined with nanoscopy, becomes a new powerful method for direct visualization and characterization of lipid raft at unprecedented resolution in living cells. The chol-N3 probe mimics cholesterol in synthetic and cellular membranes without perturbation. When combined with live-cell super-resolution microscopy, chol-N3 demonstrates the existence of cholesterol-rich nanodomains of <50 nm at the plasma membrane of resting living cells. Using this tool, the lipid membrane structure of such subdiffraction limit domains is identified, and the nanoscale spatiotemporal organization of cholesterol in the plasma membrane of living cells reveals multiple cholesterol diffusion modes at different spatial localizations. Finally, imaging across thick organ samples outlines the potential of this new method to address essential biological questions that were previously beyond reach.
AU  - Lorizate,M
AU  - Terrones,O
AU  - NietoGarai,JA
AU  - RojoBartolomé,I
AU  - Ciceri,D
AU  - Morana,O
AU  - OlazarIntxausti,J
AU  - Arboleya,A
AU  - Martin,A
AU  - Szynkiewicz,M
AU  - CallejaFelipe,M
AU  - Bernardino,de la Serna J
AU  - Contreras,F
DO  - 10.1002/smtd.202100430
EP  - 15
PY  - 2021///
SN  - 2366-9608
SP  - 1
TI  - Superresolution microscopy using a bioorthogonalbased cholesterol probe provides unprecedented capabilities for imaging nanoscale lipid heterogeneity in living cells
T2  - Small Methods
UR  - http://dx.doi.org/10.1002/smtd.202100430
UR  - https://onlinelibrary.wiley.com/doi/10.1002/smtd.202100430
UR  - http://hdl.handle.net/10044/1/90757
VL  - 5
ER  -
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