Imperial College London
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DrJanineBosse

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Howell:2017:10.1128/JCM.02464-16,
author = {Howell, KJ and Weinert, LA and Peters, SE and Wang, J and Hernandez-Garcia, J and Chaudhuri, RR and Luan, S-L and Angen, O and Aragon, V and Williamson, SM and Langford, PR and Rycroft, AN and Wren, BW and Maskell, DJ and Tucker, AW},
doi = {10.1128/JCM.02464-16},
journal = {Journal of Clinical Microbiology},
pages = {2617--2628},
title = {"Pathotyping" Multiplex PCR Assay for haemophilus parasuis: a tool for prediction of virulence},
url = {http://dx.doi.org/10.1128/JCM.02464-16},
volume = {55},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis based on a subset of 10 genes from the pangenome. A multiplex PCR (mPCR) was constructed based on these genes, the results of which were entered into the pathotyping model to yield a prediction of virulence. This new diagnostic mPCR was tested on 143 field isolates of H. parasuis that had previously been whole-genome sequenced and a further 84 isolates from the United Kingdom from cases of H. parasuis-related disease in pigs collected between 2013 and 2014. The combination of the mPCR and the pathotyping model predicted the virulence of an isolate with 78% accuracy for the original isolate collection and 90% for the additional isolate collection, providing an overall accuracy of 83% (81% sensitivity and 93% specificity) compared with that of the “current standard” of detailed clinical metadata. This new pathotyping assay has the potential to aid surveillance and disease control in addition to serotyping data.
AU  - Howell,KJ
AU  - Weinert,LA
AU  - Peters,SE
AU  - Wang,J
AU  - Hernandez-Garcia,J
AU  - Chaudhuri,RR
AU  - Luan,S-L
AU  - Angen,O
AU  - Aragon,V
AU  - Williamson,SM
AU  - Langford,PR
AU  - Rycroft,AN
AU  - Wren,BW
AU  - Maskell,DJ
AU  - Tucker,AW
DO  - 10.1128/JCM.02464-16
EP  - 2628
PY  - 2017///
SN  - 0095-1137
SP  - 2617
TI  - "Pathotyping" Multiplex PCR Assay for haemophilus parasuis: a tool for prediction of virulence
T2  - Journal of Clinical Microbiology
UR  - http://dx.doi.org/10.1128/JCM.02464-16
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000408223700009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/51448
VL  - 55
ER  -
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