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@article{Hart:2020,
author = {Hart, WS and Amrania, H and Beckley, A and Brandt, JR and Sundriyal, S and Rueda-Zubiaurre, A and Porter, AE and Aboagye, EO and Fuchter, MJ and Phillips, CC},
title = {Label-Free Chemical Nano-Imaging of Intracellular Drug Binding Sites},
url = {http://arxiv.org/abs/2005.07265v1},
year = {2020}
}

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TY  - JOUR
AB  - Optical microscopy has a diffraction limited resolution of about 250 nm.Fluorescence methods (e.g. PALM, STORM, STED) beat this, but they are stilllimited to 10 s of nm, and the images are an indirect pointillistrepresentation of only part of the original object. Here we describe a way ofcombining a sample preparation technique taken from histopathology, with aprobe-based nano-imaging technique, (s SNOM) from the world of Solid StatePhysics. This allows us to image subcellular structures optically, and at ananoscale resolution that is about 100 x better than normal microscopes. Byadding a tuneable laser source, we also demonstrate mid-infrared chemicalnano-imaging (MICHNI) in human myeloma cells and we use it to map the bindingsites of the anti cancer drug bortezomib to less than 10 zL sized intracellularcomponents. MICHNI is label free and can be used with any biological materialand drugs with specific functional chemistry. We believe that its combinationof speed, cheapness, simplicity, safety and chemical contrast promises atransformative impact across the life sciences.
AU  - Hart,WS
AU  - Amrania,H
AU  - Beckley,A
AU  - Brandt,JR
AU  - Sundriyal,S
AU  - Rueda-Zubiaurre,A
AU  - Porter,AE
AU  - Aboagye,EO
AU  - Fuchter,MJ
AU  - Phillips,CC
PY  - 2020///
TI  - Label-Free Chemical Nano-Imaging of Intracellular Drug Binding Sites
UR  - http://arxiv.org/abs/2005.07265v1
ER  -

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