Imperial College London
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Dr Jake Bundy

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3039j.bundy Website

 
 
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Location

 

E312Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sagi-Kiss:2022:10.1021/acs.jproteome.2c00030,
author = {Sagi-Kiss, V and Li, Y and Carey, MR and Grover, SJ and Siems, K and Cirulli, F and Berry, A and Musillo, C and Wilson, ID and Want, EJ and Bundy, JG},
doi = {10.1021/acs.jproteome.2c00030},
journal = {Journal of Proteome Research},
pages = {1428--1437},
title = {Ion-pairing chromatography and amine derivatization provide complementary approaches for the targeted LC-MS analysis of the polar metabolome.},
url = {http://dx.doi.org/10.1021/acs.jproteome.2c00030},
volume = {21},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Liquid chromatography coupled to mass spectrometry is a key metabolomics/metabonomics technology. Reversed-phase liquid chromatography (RPLC) is very widely used as a separation step, but typically has poor retention of highly polar metabolites. Here, we evaluated the combination of two alternative methods for improving retention of polar metabolites based on 6-aminoquinoloyl-N-hydroxysuccinidimyl carbamate derivatization for amine groups, and ion-pairing chromatography (IPC) using tributylamine as an ion-pairing agent to retain acids. We compared both of these methods to RPLC and also to each other, for targeted analysis using a triple-quadrupole mass spectrometer, applied to a library of ca. 500 polar metabolites. IPC and derivatization were complementary in terms of their coverage: combined, they improved the proportion of metabolites with good retention to 91%, compared to just 39% for RPLC alone. The combined method was assessed by analyzing a set of liver extracts from aged male and female mice that had been treated with the polyphenol compound ampelopsin. Not only were a number of significantly changed metabolites detected, but also it could be shown that there was a clear interaction between ampelopsin treatment and sex, in that the direction of metabolite change was opposite for males and females.
AU  - Sagi-Kiss,V
AU  - Li,Y
AU  - Carey,MR
AU  - Grover,SJ
AU  - Siems,K
AU  - Cirulli,F
AU  - Berry,A
AU  - Musillo,C
AU  - Wilson,ID
AU  - Want,EJ
AU  - Bundy,JG
DO  - 10.1021/acs.jproteome.2c00030
EP  - 1437
PY  - 2022///
SN  - 1535-3893
SP  - 1428
TI  - Ion-pairing chromatography and amine derivatization provide complementary approaches for the targeted LC-MS analysis of the polar metabolome.
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/acs.jproteome.2c00030
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35536659
UR  - https://pubs.acs.org/doi/10.1021/acs.jproteome.2c00030
UR  - http://hdl.handle.net/10044/1/97378
VL  - 21
ER  -
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