Imperial College London
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Dr Jake Bundy

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3039j.bundy Website

 
 
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Location

 

E312Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{La:2015:10.1111/1462-2920.13126,
author = {La, Rosa R and Behrends, V and Williams, HD and Bundy, JG and Rojo, F},
doi = {10.1111/1462-2920.13126},
journal = {Environmental Microbiology},
pages = {807--818},
title = {Influence of the Crc regulator on the hierarchical use of carbon sources from a complete medium in Pseudomonas},
url = {http://dx.doi.org/10.1111/1462-2920.13126},
volume = {18},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The Crc protein, together with the Hfq protein, participates in catabolite repression in pseudomonads, helping to coordinate metabolism. Little is known about how Crc affects the hierarchy of metabolite assimilation from complex mixtures. Using proton NMR spectroscopy, we carried out comprehensive metabolite profiling of culture supernatants (metabolic footprinting) over the course of growth of both Pseudomonas putida and P. aeruginosa, and compared the wild-type strains to deletion mutants for crc. A complex metabolite consumption hierarchy was observed, which was broadly similar between the two species, although with some important differences, for example in sugar utilisation. The order of metabolite utilisation changed upon inactivation of the crc gene, but even in the Crc-null strains some compounds were completely consumed before late metabolites were taken up. This suggests the presence of additional regulatory elements that determine the time and order of consumption of compounds. Unexpectedly, the loss of Crc led both species to excrete acetate and pyruvate as a result of unbalanced growth during exponential phase, compounds that were later consumed in stationary phase. This loss of carbon during growth helps to explain the contribution of the Crc/Hfq regulatory system to evolutionary fitness of pseudomonads.
AU  - La,Rosa R
AU  - Behrends,V
AU  - Williams,HD
AU  - Bundy,JG
AU  - Rojo,F
DO  - 10.1111/1462-2920.13126
EP  - 818
PY  - 2015///
SN  - 1462-2920
SP  - 807
TI  - Influence of the Crc regulator on the hierarchical use of carbon sources from a complete medium in Pseudomonas
T2  - Environmental Microbiology
UR  - http://dx.doi.org/10.1111/1462-2920.13126
UR  - http://hdl.handle.net/10044/1/27809
VL  - 18
ER  -
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