Imperial College London
Portrait Picture

Dr Jake Bundy

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3039j.bundy Website

 
 
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Location

 

E312Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gosztolai:2017:10.1016/j.bpj.2017.04.012,
author = {Gosztolai, A and Schumacher, J and Behrends, V and Bundy, JG and Heydenreich, F and Bennett, MH and Buck, M and Barahona, M},
doi = {10.1016/j.bpj.2017.04.012},
journal = {Biophysical journal},
pages = {2219--2230},
title = {GlnK Facilitates the Dynamic Regulation of Bacterial Nitrogen Assimilation.},
url = {http://dx.doi.org/10.1016/j.bpj.2017.04.012},
volume = {112},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Ammonium assimilation in Escherichia coli is regulated by two paralogous proteins (GlnB and GlnK), which orchestrate interactions with regulators of gene expression, transport proteins, and metabolic pathways. Yet how they conjointly modulate the activity of glutamine synthetase, the key enzyme for nitrogen assimilation, is poorly understood. We combine experiments and theory to study the dynamic roles of GlnB and GlnK during nitrogen starvation and upshift. We measure time-resolved in vivo concentrations of metabolites, total and posttranslationally modified proteins, and develop a concise biochemical model of GlnB and GlnK that incorporates competition for active and allosteric sites, as well as functional sequestration of GlnK. The model predicts the responses of glutamine synthetase, GlnB, and GlnK under time-varying external ammonium level in the wild-type and two genetic knock-outs. Our results show that GlnK is tightly regulated under nitrogen-rich conditions, yet it is expressed during ammonium run-out and starvation. This suggests a role for GlnK as a buffer of nitrogen shock after starvation, and provides a further functional link between nitrogen and carbon metabolisms.
AU  - Gosztolai,A
AU  - Schumacher,J
AU  - Behrends,V
AU  - Bundy,JG
AU  - Heydenreich,F
AU  - Bennett,MH
AU  - Buck,M
AU  - Barahona,M
DO  - 10.1016/j.bpj.2017.04.012
EP  - 2230
PY  - 2017///
SN  - 0006-3495
SP  - 2219
TI  - GlnK Facilitates the Dynamic Regulation of Bacterial Nitrogen Assimilation.
T2  - Biophysical journal
UR  - http://dx.doi.org/10.1016/j.bpj.2017.04.012
UR  - http://hdl.handle.net/10044/1/48138
VL  - 112
ER  -
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