Publications
98 results found
de Groot R, Ryu J-Y, Jaring M, et al., 2011, VWF proteolysis by adamts13 is dependent on cooperation between the ADAMTS13 cysteine-rich domain loop Q456-Q478 and the spacer domain, Publisher: WILEY-BLACKWELL, Pages: 307-307, ISSN: 1538-7933
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- Citations: 1
Ahnstroem J, Andersson HM, Canis K, et al., 2011, Activated protein C cofactor function of protein S: a novel role for a γ-carboxyglutamic acid residue, BLOOD, Vol: 117, Pages: 6685-6693, ISSN: 0006-4971
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- Citations: 31
Crawley JTB, Gonzalez-Porras JR, Lane DA, 2011, The Coagulation Cascade and Its Regulation, TEXTBOOK OF PULMONARY VASCULAR DISEASE, Editors: Yuan, Garcia, Hales, Rich, Archer, West, Publisher: SPRINGER, Pages: 357-370, ISBN: 978-0-387-87428-9
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- Citations: 1
Smith TJ, Yamamoto K, Kurata M, et al., 2010, Differential expression of Toll-like receptors in follicular lymphoma, diffuse large B-cell lymphoma and peripheral T-cell lymphoma, EXPERIMENTAL AND MOLECULAR PATHOLOGY, Vol: 89, Pages: 284-290, ISSN: 0014-4800
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- Citations: 21
de Groot R, Lane DA, Crawley JTB, 2010, The ADAMTS13 metalloprotease domain: roles of subsites in enzyme activity and specificity, BLOOD, Vol: 116, Pages: 3064-3072, ISSN: 0006-4971
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- Citations: 33
Luken BM, Winn LYN, Emsley J, et al., 2010, The importance of vicinal cysteines, C1669 and C1670, for von Willebrand factor A2 domain function, BLOOD, Vol: 115, Pages: 4910-4913, ISSN: 0006-4971
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- Citations: 40
Andersson HM, Arantes MJ, Crawley JTB, et al., 2010, Activated protein C cofactor function of protein S: a critical role for Asp95 in the EGF1-like domain, BLOOD, Vol: 115, Pages: 4878-4885, ISSN: 0006-4971
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- Citations: 25
Pos W, Crawley JTB, Fijnheer R, et al., 2010, An autoantibody epitope comprising residues R660, Y661, and Y665 in the ADAMTS13 spacer domain identifies a binding site for the A2 domain of VWF, BLOOD, Vol: 115, Pages: 1640-1649, ISSN: 0006-4971
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- Citations: 99
Crawley JTB, de Groot R, Luken BM, 2009, Circulating ADAMTS-13-von Willebrand factor complexes: an enzyme on demand, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 7, Pages: 2085-2087, ISSN: 1538-7933
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- Citations: 7
de Groot R, Lane DA, Crawley JTB, 2009, ADAMTS13 metalloprotease domain subsites control cleavage site specificity, Publisher: ELSEVIER SCIENCE INC, Pages: 963-963, ISSN: 1538-7933
de Groot R, Ramroop N, Bardhan A, et al., 2009, Identification of a functional exosite in the ADAMTS13 disintegrin-like domain, Publisher: ELSEVIER SCIENCE INC, Pages: 963-963, ISSN: 1538-7933
Luken BM, Pos W, Voorberg J, et al., 2009, TTP autoantibodies define a VWF binding site in the ADAMTS13 spacer domain, Publisher: ELSEVIER SCIENCE INC, Pages: 58-58, ISSN: 1538-7933
Andersson HM, Arantes MJ, Crawley JTB, et al., 2009, Activated protein C cofactor function of protein S: a critical role for the EGF1-like domain, Publisher: ELSEVIER SCIENCE INC, Pages: 37-37, ISSN: 1538-7933
Luken BM, Winn LYN, Emsley J, et al., 2009, Stabilisation of the VWF A2 domain by introduction of a disulphide bond prevents binding to and cleavage by ADAMTS13, Publisher: ELSEVIER SCIENCE INC, Pages: 346-346, ISSN: 1538-7933
de Groot R, Bardhan A, Ramroop N, et al., 2009, Essential role of the disintegrin-like domain in ADAMTS13 function, BLOOD, Vol: 113, Pages: 5609-5616, ISSN: 0006-4971
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- Citations: 63
Crawley JTB, Gonzalez-Porras JR, Lane DA, 2009, Textbook of Pulmonary Vascular Disease, Textbook of Pulmonary Vascular Disease, Editors: Yuan, Garcia, Hales, Publisher: Springer Verlag, ISBN: 9780387874289
Konstantinou K, Yamamoto K, Ishibashi F, et al., 2009, Angiogenic mediators of the angiopoietin system are highly expressed by CD10-positive lymphoma cells in angioimmunoblastic T-cell lymphoma, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 144, Pages: 696-704, ISSN: 0007-1048
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- Citations: 13
Gardner MD, Chion C, de Groot R, et al., 2009, A functional calcium-binding site in the metalloprotease domain of ADAMTS13, Blood, Vol: Vol. 113, Pages: 1149-1157
Crawley JTB, Efthymiou M, 2008, Cytoprotective effect of activated protein C: specificity of PAR-1 signaling, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 6, Pages: 951-953, ISSN: 1538-7933
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- Citations: 3
Crawley JTB, Lane DA, Woodward M, et al., 2008, Evidence that high von Willebrand factor and low ADAMTS-13 levels independently increase the risk of a non-fatal heart attack, Journal of Thrombosis and Haemostasis, Vol: 6, Pages: 583-588, ISSN: 1538-7933
Background: A disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS-13) may influence von Willebrand factor (VWF) levels and consequently the risk of myocardial infarction (MI). Moreover, ADAMTS-13 influences hemostatic plug formation in mouse models. We therefore studied their associations in the Glasgow MI Study (GLAMIS). Methods and results: We measured ADAMTS-13 and VWF antigen levels by ELISAs in stored plasma from a case–control study of 466 MI cases and 484 age- and sex-matched controls from the same north Glasgow population. There was no correlation between ADAMTS-13 and VWF levels in cases or controls. ADAMTS-13 levels correlated positively with serum cholesterol and triglycerides and body mass index, and negatively with high-density lipoprotein-cholesterol. VWF levels correlated with age, fibrinogen and C-reactive protein. In multivariable analyses including risk factors, VWF correlated positively with risk of MI, and ADAMTS-13 correlated negatively with risk of MI. These associations were independent of each other. The association of ADAMTS-13 with risk of MI was observed only in multivariable analysis. Conclusions: VWF and ADAMTS-13 levels were not associated in this study, and showed associations with MI risk in opposite directions but of similar strength. The association of ADAMTS-13 with MI is influenced by lipid levels, and consequently requires further investigation.
Crawley JTB, Lane DA, 2008, The haemostatic role of tissue factor pathway inhibitor, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 28, Pages: 233-242, ISSN: 1079-5642
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- Citations: 104
Camilleri RS, Cohen H, Mackie IJ, et al., 2008, Prevalence of the ADAMTS-13 missense mutation R1060W in late onset adult thrombotic thrombocytopenic purpura., J Thromb Haemost, Vol: 6, Pages: 331-338
BACKGROUND: Thrombotic thrombocytopenic purpura (TTP) is most commonly associated with deficiency or inhibition of von Willebrand factor-cleaving protease (ADAMTS-13) activity. ADAMTS-13 mutations and polymorphisms have been reported in childhood congenital TTP, but their significance in adult onset TTP remains unclear. OBJECTIVES: We sought to identify common ADAMTS-13 mutations in adults with late onset TTP and to investigate whether they may predispose acute clinical episodes of the disorder in adulthood. PATIENTS/METHODS/RESULTS: We detected a missense mutation (C3178T) in exon 24 of ADAMTS-13 in 6/53 (11.3%) adult onset TTP patients, but no normal controls (n = 100). Three of the patients had pregnancy-associated TTP; three had chronic relapsing acute idiopathic TTP. C3178T encodes an arginine to tryptophan (R1060W) substitution in the TSP1-7 domain of ADAMTS-13. In vitro expression of mutant and wild-type ADAMTS-13 demonstrated that R1060W caused severe intracellular retention of ADAMTS-13 (<5% secretion) without affecting its metalloprotease activity. One homozygous and five heterozygous patients were identified. No other causative mutations were discovered, yet all six patients had ADAMTS-13 activity levels <5% at presentation (normal: 66-126%). Antibodies/inhibitors to ADAMTS-13 were detected in three/five heterozygous patients, and all six patients had subnormal antigen levels. Six asymptomatic first-degree relatives, including those of two probands with antibodies, were also heterozygous for C3178T; all but one had subnormal ADAMTS-13 activity. CONCLUSION: The high prevalence of R1060W ADAMTS-13 in adult onset TTP, together with its absence in childhood congenital TTP cases reported elsewhere, suggests it may be a factor in the development of late onset TTP.
Crawley JTB, 2007, Multiple roles of the endothelial cell protein C receptor, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 5, Pages: 1813-1816, ISSN: 1538-7933
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- Citations: 3
Crawley JTB, Zanardelli S, Chion CKNK, et al., 2007, The central role of thrombin in hemostasis, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 5, Pages: 95-101, ISSN: 1538-7933
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- Citations: 238
Lam JK, Chion CKNK, Zanardelli S, et al., 2007, Further characterization of ADAMTS-13 inactivation by thrombin, Journal of Thrombosis and Haemostasis, Vol: 5, Pages: 1010-1018, ISSN: 1538-7933
Background: The multimeric size and platelet-tethering function of von Willebrand factor (VWF) are modulated by the plasma metalloprotease, a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS-13). In vitro ADAMTS-13 is susceptible to proteolytic inactivation by thrombin. Objectives: In this study, we aimed to characterize the inactivation of ADAMTS-13 by thrombin and to assess its physiological significance. Methods and results: By N-terminal sequencing of cleavage products, and by mutagenesis, we identified the principal thrombin cleavage sites in ADAMTS-13 as R257 and R1176. Using a library of 76 thrombin mutants, we highlighted the functional importance of exosite I on thrombin in the proteolysis of ADAMTS-13. Proteolysis of ADAMTS-13 by thrombin caused an 8-fold reduction in its affinity for VWF that contributed to its loss of VWF-cleaving function. Intriguingly, thrombin-cleaved ADAMTS-13 both bound and proteolyzed a short recombinant VWF A2 domain substrate (VWF115) normally. Following activation of coagulation in normal plasma, endogenous ADAMTS-13, but not added ADAMTS-13, appeared resistant to coagulation-induced fragmentation. An estimation of the Km for ADAMTS-13 proteolysis by thrombin was appreciably higher than the physiological concentration of ADAMTS-13. This was corroborated by the comparatively low affinity of ADAMTS-13 for thrombin (KD 95 nm). Conclusions: Together, our data suggest that ADAMTS-13 is protected from rapid proteolytic inactivation by thrombin in normal plasma. Whether this remains the case under pathological situations involving elevated/sustained generation of thrombin remains unclear.
Chion CKNK, Doggen CJM, Crawley JTB, et al., 2007, ADAMTS13 and von Willebrand factor and the risk of myocardial infarction in men, BLOOD, Vol: 109, Pages: 1998-2000, ISSN: 0006-4971
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- Citations: 103
Mollica LR, Crawley JTB, Liu K, et al., 2006, Role of a 5′-enhancer in the transcriptional regulation of the human endothelial cell protein C receptor gene, BLOOD, Vol: 108, Pages: 1251-1259, ISSN: 0006-4971
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- Citations: 20
Zanardelli S, Crawley JTB, Chion CKNCK, et al., 2006, ADAMTS13 substrate recognition of von Willebrand factor A2 domain, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 1555-1563
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- Citations: 69
O'Donnell JS, McKinnon TAJ, Crawley JTB, et al., 2005, Bombay phenotype is associated with reduced plasma-VWF levels and an increased susceptibility to ADAMTS13 proteolysis, BLOOD, Vol: 106, Pages: 1988-1991, ISSN: 0006-4971
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- Citations: 79
Crawley JTB, Lam JK, Rance JB, et al., 2005, Proteolytic inactivation of ADAMTS13 by thrombin and plasmin, BLOOD, Vol: 105, Pages: 1085-1093, ISSN: 0006-4971
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- Citations: 170
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