Imperial College London
Alternate

ProfessorJimCrawley

Faculty of Medicine, Department of Immunology and Inflammation

Professor of Haemostasis
 
 
 
//

Contact

 

+44 (0)20 3313 2297j.crawley Website

 
 
//

Location

 

5S5aCommonwealth BuildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Ferreira:2020:10.1038/s41598-020-73005-3,
author = {Ferreira, PM and Bozbas, E and Tannetta, SD and Alroqaiba, N and Zhou, R and Crawley, JTB and Gibbins, JM and Jones, CI and Ahnström, J and Yaqoob, P},
doi = {10.1038/s41598-020-73005-3},
journal = {Scientific Reports},
title = {Mode of induction of platelet-derived extracellular vesicles is a critical determinant of their phenotype and function},
url = {http://dx.doi.org/10.1038/s41598-020-73005-3},
volume = {10},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Platelet-derived extracellular vesicles (PDEVs) are the most abundant amongst all types of EVs in the circulation. However, the mechanisms leading to PDEVs release, their role in coagulation and phenotypic composition are poorly understood. PDEVs from washed platelets were generated using different stimuli and were characterised using nanoparticle tracking analysis. Procoagulant properties were evaluated by fluorescence flow cytometry and calibrated automated thrombography. EVs from plasma were isolated and concentrated using a novel protocol involving a combination of size exclusion chromatography and differential centrifugation, which produces pure and concentrated EVs. Agonist stimulation enhanced PDEV release, but did not alter the average size of EVs compared to those produced by unstimulated platelets. Agonist stimulation led to lower negatively-charged phospholipid externalization in PDEVs, which was reflected in the lower procoagulant activity compared to those generated without agonist stimulation. Circulating EVs did not have externalized negatively-charged phospholipids. None of the 4 types of EVs presented tissue factor. The mechanism by which PDEV formation is induced is a critical determinant of its phenotype and function. Importantly, we have developed methods to obtain clean, concentrated and functional EVs derived from platelet-free plasma and washed platelets, which can be used to provide novel insight into their biological functions.
AU  - Ferreira,PM
AU  - Bozbas,E
AU  - Tannetta,SD
AU  - Alroqaiba,N
AU  - Zhou,R
AU  - Crawley,JTB
AU  - Gibbins,JM
AU  - Jones,CI
AU  - Ahnström,J
AU  - Yaqoob,P
DO  - 10.1038/s41598-020-73005-3
PY  - 2020///
SN  - 2045-2322
TI  - Mode of induction of platelet-derived extracellular vesicles is a critical determinant of their phenotype and function
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-020-73005-3
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33093473
UR  - http://hdl.handle.net/10044/1/83719
VL  - 10
ER  -
Download