Imperial College London
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DrJoseCortes Lopez

Faculty of Engineering, Department of Bioengineering

 
 
 
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Contact

 

+44 (0)7944 338 144j.e.corteslopez Website

 
 
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Location

 

B-122Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haining:2018:10.1371/journal.pbio.2005599,
author = {Haining, AWM and Rahikainen, R and Cortes, E and Lachowski, D and Rice, A and von, Essen M and Hytonen, VP and Del, Rio Hernandez A},
doi = {10.1371/journal.pbio.2005599},
journal = {PLoS Biology},
pages = {1--20},
title = {Mechanotransduction in talin through the interaction of the R8 domain with DLC1},
url = {http://dx.doi.org/10.1371/journal.pbio.2005599},
volume = {16},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The mechanical unfolding of proteins is a cellular mechanism for force transduction with potentially broad implications in cell fate. Despite this, the mechanism by which protein unfolding elicits differential downstream signalling pathways remains poorly understood. Here, we used protein engineering, atomic force microscopy, and biophysical tools to delineate how protein unfolding controls cell mechanics. Deleted in liver cancer 1 (DLC1) is a negative regulator of Ras homolog family member A (RhoA) and cell contractility that regulates cell behaviour when localised to focal adhesions bound to folded talin. Using a talin mutant resistant to force-induced unfolding of R8 domain, we show that talin unfolding determines DLC1 downstream signalling and, consequently, cell mechanics. We propose that this new mechanism of mechanotransduction may have implications for a wide variety of associated cellular processes.
AU  - Haining,AWM
AU  - Rahikainen,R
AU  - Cortes,E
AU  - Lachowski,D
AU  - Rice,A
AU  - von,Essen M
AU  - Hytonen,VP
AU  - Del,Rio Hernandez A
DO  - 10.1371/journal.pbio.2005599
EP  - 20
PY  - 2018///
SN  - 1544-9173
SP  - 1
TI  - Mechanotransduction in talin through the interaction of the R8 domain with DLC1
T2  - PLoS Biology
UR  - http://dx.doi.org/10.1371/journal.pbio.2005599
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000440397800020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2005599
UR  - http://hdl.handle.net/10044/1/66860
VL  - 16
ER  -
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