Imperial College London
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DR JAMES M. FLANAGAN

Faculty of Medicine, Department of Surgery & Cancer

Reader in Epigenetics
 
 
 
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Contact

 

+44 (0)20 7594 2127j.flanagan

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Beetch:2019:10.1002/mnfr.201801386,
author = {Beetch, M and Lubecka, K and Shen, K and Flower, K and Harandi-Zadeh, S and Suderman, M and Flanagan, JM and Stefanska, B},
doi = {10.1002/mnfr.201801386},
journal = {Mol Nutr Food Res},
pages = {e1801386--e1801386},
title = {Stilbenoid-Mediated Epigenetic Activation of Semaphorin 3A in Breast Cancer Cells Involves Changes in Dynamic Interactions of DNA with DNMT3A and NF1C Transcription Factor.},
url = {http://dx.doi.org/10.1002/mnfr.201801386},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - SCOPE: Loci-specific increase in DNA methylation occurs in cancer and may underlie gene silencing. It is investigated whether dietary stilbenoids, resveratrol, and pterostilbene exert time-dependent effects on DNA methylation patterns and specifically methylation-silenced tumor suppressor genes in breast cancer cells. METHODS AND RESULTS: Following genome-wide DNA methylation analysis with Illumina-450K, changes characteristic of early and late response to stilbenoids are identified. Interestingly, often the same genes but at different CpG loci, the same gene families, or the same functional gene categories are affected. CpG loci that lose methylation in exposed cells correspond to genes functionally associated with cancer suppression. There is a group of genes, including SEMA3A, at which the magnitude of hypomethylation in response to stilbenoids rises with increasing invasive potential of cancer cells. Decreased DNA methylation at SEMA3A promoter and concomitant gene upregulation coincide with increased occupancy of active histone marks. Open chromatin upon exposure to stilbenoids may be linked to decreased DNMT3A binding followed by increased NF1C transcription factor occupancy. Sequestration of DNMT3A is possibly a result of stilbenoid-mediated increase in SALL3 expression, which was previously shown to bind and inhibit DNMT3A activity. CONCLUSIONS: The findings define mechanistic players in stilbenoid-mediated epigenetic reactivation of genes suppressing cancer.
AU  - Beetch,M
AU  - Lubecka,K
AU  - Shen,K
AU  - Flower,K
AU  - Harandi-Zadeh,S
AU  - Suderman,M
AU  - Flanagan,JM
AU  - Stefanska,B
DO  - 10.1002/mnfr.201801386
EP  - 1801386
PY  - 2019///
SP  - 1801386
TI  - Stilbenoid-Mediated Epigenetic Activation of Semaphorin 3A in Breast Cancer Cells Involves Changes in Dynamic Interactions of DNA with DNMT3A and NF1C Transcription Factor.
T2  - Mol Nutr Food Res
UR  - http://dx.doi.org/10.1002/mnfr.201801386
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31327173
ER  -
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