Imperial College London
Alternate

DrJohnHeap

Faculty of Natural Sciences, Department of Life Sciences

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5355j.heap

 
 
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Location

 

Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kelly:2018:10.1021/acssynbio.7b00435,
author = {Kelly, CL and Taylor, GM and Hitchcock, A and Torres-Méndez, A and Heap, JT},
doi = {10.1021/acssynbio.7b00435},
journal = {ACS Synth Biol},
pages = {1056--1066},
title = {A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria.},
url = {http://dx.doi.org/10.1021/acssynbio.7b00435},
volume = {7},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer.
AU  - Kelly,CL
AU  - Taylor,GM
AU  - Hitchcock,A
AU  - Torres-Méndez,A
AU  - Heap,JT
DO  - 10.1021/acssynbio.7b00435
EP  - 1066
PY  - 2018///
SP  - 1056
TI  - A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria.
T2  - ACS Synth Biol
UR  - http://dx.doi.org/10.1021/acssynbio.7b00435
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29544054
UR  - http://hdl.handle.net/10044/1/58410
VL  - 7
ER  -
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