Imperial College London
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Dr James W. Hindley

Faculty of Natural Sciences, Department of Chemistry

Research Associate/Research Group Manager
 
 
 
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Contact

 

+44 (0)20 7589 5111 ext 55816j.hindley14 Website

 
 
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Location

 

207Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Strutt:2021:10.1039/d0sc03889j,
author = {Strutt, R and Hindley, JW and Gregg, J and Booth, PJ and Harling, JD and Law, RV and Friddin, MS and Ces, O},
doi = {10.1039/d0sc03889j},
journal = {Chemical Science},
pages = {2138--2145},
title = {Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry},
url = {http://dx.doi.org/10.1039/d0sc03889j},
volume = {12},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between membrane separated compartments. This has been demonstrated with droplet interface bilayers (DIBs) using several different membrane proteins combined with electrical, mechanical, and/or chemical activators. Here we report the activation of the bacterial mechanosensitive channel of large conductance (MscL) in a dioleoylphosphatidylcholine:dioleoylphosphatidylglycerol DIB by controlling membrane asymmetry. We show using electrical measurements that the incorporation of lysophosphatidylcholine (LPC) into one of the bilayer leaflets triggers MscL gating in a concentration-dependent manner, with partial and full activation observed at 10 and 15 mol% LPC respectively. Our findings could inspire the design of new minimal tissues where flux pathways are dynamically defined by lipid composition.
AU  - Strutt,R
AU  - Hindley,JW
AU  - Gregg,J
AU  - Booth,PJ
AU  - Harling,JD
AU  - Law,RV
AU  - Friddin,MS
AU  - Ces,O
DO  - 10.1039/d0sc03889j
EP  - 2145
PY  - 2021///
SN  - 2041-6520
SP  - 2138
TI  - Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry
T2  - Chemical Science
UR  - http://dx.doi.org/10.1039/d0sc03889j
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/SC/D0SC03889J#!divAbstract
UR  - http://hdl.handle.net/10044/1/85183
VL  - 12
ER  -
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