Imperial College London
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Dr James W. Hindley

Faculty of Natural Sciences, Department of Chemistry

Research Associate/Research Group Manager
 
 
 
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Contact

 

+44 (0)20 7589 5111 ext 55816j.hindley14 Website

 
 
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Location

 

207Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Zhang:2020:rs.3.rs-37639/v1,
author = {Zhang, S and Contini, C and Hindley, J and Bolognesi, G and Elani, Y and Ces, O},
doi = {rs.3.rs-37639/v1},
title = {Engineering motile aqueous phase-separated droplets via liposome stabilisation},
url = {http://dx.doi.org/10.21203/rs.3.rs-37639/v1},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title>        <jats:p>There are increasing efforts to engineer functional compartments that mimic aspects of cellular behaviour in a drive to construct an artificial cell from the bottom-up. One behaviour that is receiving particular attention is motility, due to its biotechnological potential and the fact that movement of discrete cells is a ubiquitous feature of living systems. Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems. However, most of these systems are unsuitable for biological applications due to issues with biocompatibility caused by the presence of oil phases. Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilized cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration. We demonstrate that the compartments are capable of negative chemotaxis: if water is introduced into the emulsion system, these droplets can respond through directional motion away from PEG in the continuous phase and down to the polymer gradient with a velocity change proportional to the rearrangement of liposome stabilisers in the PEG/dextran interface. The biocompatibility, motility and partitioning abilities of this novel droplet system offers new directions to pursue research in motion-related biological processes.</jats:p>
AU  - Zhang,S
AU  - Contini,C
AU  - Hindley,J
AU  - Bolognesi,G
AU  - Elani,Y
AU  - Ces,O
DO  - rs.3.rs-37639/v1
PY  - 2020///
TI  - Engineering motile aqueous phase-separated droplets via liposome stabilisation
UR  - http://dx.doi.org/10.21203/rs.3.rs-37639/v1
ER  -
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