Imperial College London
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DrJunIshihara

Faculty of Engineering, Department of Bioengineering

Lecturer
 
 
 
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Contact

 

j.ishihara Website CV

 
 
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Location

 

86 Woodlane London W12 0BZSir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ishihara:2018:10.1038/s41467-018-04525-w,
author = {Ishihara, J and Ishihara, A and Fukunaga, K and Sasaki, K and White, MJV and Briquez, PS and Hubbell, JA},
doi = {10.1038/s41467-018-04525-w},
journal = {Nature Communications},
pages = {1--14},
title = {Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing},
url = {http://dx.doi.org/10.1038/s41467-018-04525-w},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Laminin, as a key component of the basement membrane extracellular matrix (ECM), regulates tissue morphogenesis. Here, we show that multiple laminin isoforms promiscuously bind to growth factors (GFs) with high affinity, through their heparin-binding domains (HBDs) located in the α chain laminin-type G (LG) domains. These domains also bind to syndecan cell-surface receptors, promoting attachment of fibroblasts and endothelial cells. We explore the application of these multifunctional laminin HBDs in wound healing in the type-2 diabetic mouse. We demonstrate that covalent incorporation of laminin HBDs into fibrin matrices improves retention of GFs and significantly enhances the efficacy of vascular endothelial cell growth factor (VEGF-A165) and platelet-derived growth factor (PDGF-BB) in promoting wound healing in vivo, under conditions where the GFs alone in fibrin are inefficacious. This laminin HBD peptide may be clinically useful by improving biomaterial matrices as both GF reservoirs and cell scaffolds, leading to effective tissue regeneration.
AU  - Ishihara,J
AU  - Ishihara,A
AU  - Fukunaga,K
AU  - Sasaki,K
AU  - White,MJV
AU  - Briquez,PS
AU  - Hubbell,JA
DO  - 10.1038/s41467-018-04525-w
EP  - 14
PY  - 2018///
SN  - 2041-1723
SP  - 1
TI  - Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-018-04525-w
UR  - https://www.nature.com/articles/s41467-018-04525-w
UR  - http://hdl.handle.net/10044/1/83371
VL  - 9
ER  -
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