Publications
272 results found
Alexander J, Perdones-Montero A, Scott A, et al., 2017, A PROSPECTIVE MULTI-NATIONAL STUDY OF THE COLORECTAL CANCER MUCOSAL MICROBIOME REVEALS SPECIFIC TAXONOMIC CHANGES INDICATIVE OF DISEASE STAGE AND PROGNOSIS, Annual General Meeting of the British-Society-of-Gastroenterology (BSG), Publisher: BMJ PUBLISHING GROUP, Pages: A32-A33, ISSN: 0017-5749
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Pawa N, Clift AK, Osmani H, et al., 2017, Surgical management of patients with neuroendocrine neoplasms of the appendix: appendectomy or more?, Neuroendocrinology, Vol: 106, Pages: 242-251, ISSN: 0028-3835
Background: Appendiceal neuroendocrine neoplasms (ANEN) are mostly indolent tumours treated effectively with simple appendectomy. However, controversy exists regarding the necessity of oncologic right hemicolectomy (RH) in patients with histologic features suggestive of more aggressive disease. We assess the effects of current guidelines in selecting the surgical strategy (appendectomy or RH) for the management of ANEN. Methods/Aims: This is a retrospective review of all ANEN cases treated over a 14-year period at 3 referral centres and their management according to consensus guidelines of the European and the North American Neuroendocrine Tumor Societies (ENETS and NANETS, respectively). The operation performed, the tumour stage and grade, the extent of residual disease, and the follow-up outcomes were evaluated. Results: Of 14,850 patients who had appendectomies, 215 (1.45%) had histologically confirmed ANEN. Four patients had synchronous non-ANEN malignancies. One hundred and ninety-three patients had index appendectomy. Seventeen patients (7.9%) had lymph node metastases within the mesoappendix. Forty-nine patients underwent RH after appendectomy. The percentages of 30-day morbidity and mortality after RH were 2 and 0%, respectively. Twelve patients (24.5%) receiving completion RH were found to have lymph node metastases. Two patients had liver metastases, both of them synchronous. The median follow-up was 38.5 months (range 1-143). No patient developed disease recurrence. Five- and 10-year overall survival for all patients with ANEN as the only malignancy was both 99.05%. Conclusions: The current guidelines appear effective in identifying ANEN patients at risk of harbouring nodal disease, but they question the oncological relevance of ANEN lymph node metastases. RH might present an overtreatment for a number of patients with ANEN.
Alexander J, Poynter L, Scott A, et al., 2017, A prospective multi-centered analysis of the rectal cancer mucosal microbiome during neoadjuvant long course chemoradiotherapy, Annual Scientific Meeting of the American Society of Colon and Rectal Surgeons, Publisher: Lippincott, Williams & Wilkins, Pages: E118-E118, ISSN: 0012-3706
Scott A, Lewis M, Gomez-Romero M, et al., 2017, LONGITUDINAL ANALYSIS OF URINARY METABOLIC PHENOTYPE AFTER COLORECTAL RESECTION DEMONSTRATE TEMPORAL EVOLUTION., Annual Scientific Meeting of the American-Society-of-Colon-and-Rectal-Surgeons, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: E121-E121, ISSN: 0012-3706
Martin G, Kinross J, Hankin C, 2017, Effective cybersecurity is fundamental to patient safety, British Medical Journal, Vol: 357, ISSN: 1468-5833
Wolfer AM, Scott AJ, Rueb C, et al., 2017, Longitudinal analysis of serum oxylipin profile as a novel descriptor of the inflammatory response to surgery, JOURNAL OF TRANSLATIONAL MEDICINE, Vol: 15, ISSN: 1479-5876
Background:Oxylipins are potent lipid mediators demonstrated to initiate and regulate inflammation yet little is known regarding their involvement in the response to surgical trauma. As key modulators of the inflammatory response, oxylipins have the potential to provide novel insights into the physiological response to surgery and the pathophysiology of post-operative complications. We aimed to investigate the effects of major surgery on longitudinal oxylipin profile.Methods:Adults patients undergoing elective laparoscopic or open colorectal resections were included. Primary outcomes were serum oxylipin profile quantified by ultra high-performance liquid chromatography-mass spectrometry, serum white cell count and C-reactive protein concentration. Serum samples were taken at three time-points: pre-operative (day zero), early post-operative (day one) and late post-operative (day four/five).Results:Some 55 patients were included, of which 33 (60%) underwent surgery that was completed laparoscopically. Pre-operative oxylipin profiles were characterised by marked heterogeneity but surgery induced a common shift resulting in more homogeneity at the early post-operative time-point. By the late post-operative phase, oxylipin profiles were again highly variable. This evolution was driven by time-dependent changes in specific oxylipins. Notably, the levels of several oxylipins with anti-inflammatory properties (15-HETE and four regioisomers of DHET) were reduced at the early post-operative point before returning to baseline by the late post-operative period. In addition, levels of the pro-inflammatory 11-HETE rose in the early post-operative phase while levels of anti-thrombotic mediators (9-HODE and 13-HODE) fell; concentrations of all three oxylipins then remained fairly static from early to late post-operative phases. Compared to those undergoing laparoscopic surgery, patients undergoing open surgery had lower levels of some anti-inflammatory oxylipins (8,9-DHET and 17-HD
Kinross JM, Alexander J, Perdones-Monter A, et al., 2017, A Prospective Multi-National Study of the Colorectal Cancer Mucosal Microbiome Reveals Specific Taxonomic Changes Indicative of Disease Stage and Prognosis, DDW
Poynter LR, Veselkov K, Galea D, et al., 2017, Network-driven analytics of published tissue-based biomarkers to predict response to neoadjuvant therapy in rectal cancer, Annual Meeting of the American-Association-for-Cancer-Research (AACR), Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472
Scott A, Alexander JL, Marchesi J, et al., 2017, MODIFICATION OF THE APPENDIX MICROBIOME IN ACUTE APPENDICITIS AND COLON ADENOCARCINOMA, Digestive Disease Week (DDW), Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S1228-S1228, ISSN: 0016-5085
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- Citations: 3
Kinross J, 2017, Race-specific differences in mucosal sulfidogenic bacteria modify colon cancer risk., Gut
Alexander JL, Wilson ID, Teare J, et al., 2017, Gut microbiota modulation of chemotherapy efficacy and toxicity., Nature Reviews Gastroenterology and Hepatology, Vol: 14, Pages: 356-365, ISSN: 1759-5045
Evidence is growing that the gut microbiota modulates the host response to chemotherapeutic drugs, with three main clinical outcomes: facilitation of drug efficacy; abrogation and compromise of anticancer effects; and mediation of toxicity. The implication is that gut microbiota are critical to the development of personalized cancer treatment strategies and, therefore, a greater insight into prokaryotic co-metabolism of chemotherapeutic drugs is now required. This thinking is based on evidence from human, animal and in vitro studies that gut bacteria are intimately linked to the pharmacological effects of chemotherapies (5-fluorouracil, cyclophosphamide, irinotecan, oxaliplatin, gemcitabine, methotrexate) and novel targeted immunotherapies such as anti-PD-L1 and anti-CLTA-4 therapies. The gut microbiota modulate these agents through key mechanisms, structured as the 'TIMER' mechanistic framework: Translocation, Immunomodulation, Metabolism, Enzymatic degradation, and Reduced diversity and ecological variation. The gut microbiota can now, therefore, be targeted to improve efficacy and reduce the toxicity of current chemotherapy agents. In this Review, we outline the implications of pharmacomicrobiomics in cancer therapeutics and define how the microbiota might be modified in clinical practice to improve efficacy and reduce the toxic burden of these compounds.
Inglese P, McKenzie JS, Mroz A, et al., 2017, Deep learning and 3D-DESI imaging reveal the hidden metabolic heterogeneity of cancer, Chemical Science, Vol: 8, Pages: 3500-3511, ISSN: 2041-6539
Visual inspection of tumour tissues does not reveal the complex metabolic changes that differentiate cancer and its sub-types from healthy tissues. Mass spectrometry imaging, which quantifies the underlying chemistry, represents a powerful tool for the molecular exploration of tumour tissues. A 3-dimensional topological description of the chemical properties of the tumour permits the formulation of hypotheses about the biological composition and interactions and the possible causes of its heterogeneous structure. The large amount of information contained in such datasets requires powerful tools for its analysis, visualisation and interpretation. Linear methods for unsupervised dimensionality reduction, such as PCA, are inadequate to capture the complex non-linear relationships present in these data. For this reason, a deep unsupervised neural network based technique, parametric t-SNE, is adopted to map a 3D-DESI-MS dataset from a human colorectal adenocarcinoma biopsy onto a 2-dimensional manifold. This technique allows the identification of clusters not visible with linear methods. The unsupervised clustering of the tumour tissue results in the identification of sub-regions characterised by the abundance of identified metabolites, making possible the formulation of hypotheses to account for their significance and the underlying biological heterogeneity in the tumour.
Diederen K, Li JV, Kinross J, et al., 2017, Exclusive enteral nutrition mediates gut metabolic changes in children with Crohn’s disease, Digestive Disease Week
Hund-Rinke K, Baun A, Cupi D, et al., 2016, Regulatory ecotoxicity testing of nanomaterials - proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles, NANOTOXICOLOGY, Vol: 10, Pages: 1442-1447, ISSN: 1743-5390
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- Citations: 86
Alexander J, Mroz A, Perdones-Monteiro A, et al., 2016, OP165 Targeted chemical analysis of the colon cancer microbiome using desorption electrospray ionisation mass spectrometry imaging (DESI-MSI), UEGW 2016, Publisher: Sage Publications, Pages: A67-A68
Mason SE, Kinross JM, Hendricks J, et al., 2016, Postoperative hypothermia and surgical site infection following peritoneal insufflation with warm, humidified carbon dioxide during laparoscopic colorectal surgery: a cohort study with cost-effectiveness analysis., Surgical Endoscopy, Vol: 31, Pages: 1923-1929, ISSN: 0930-2794
BACKGROUND: Surgical Site Infection (SSI) occurs in 9 % of laparoscopic colorectal surgery. Warming and humidifying carbon dioxide (CO2) used for peritoneal insufflation may protect against SSI by avoiding postoperative hypothermia (itself a risk factor for SSI). This study aimed to assess the impact of CO2 conditioning on postoperative hypothermia and SSI and to perform a cost-effectiveness analysis. METHODS: A retrospective cohort study of patients undergoing elective laparoscopic colorectal resection was performed at a single UK specialist centre. The control group (n = 123) received peritoneal insufflation with room temperature, dry CO2, whereas the intervention group (n = 123) received warm, humidified CO2 (using HumiGard™, Fisher & Paykel Healthcare). The outcomes were postoperative hypothermia, SSI and costs. Multivariate analysis was performed. RESULTS: A total of 246 patients were included in the study. The mean age was 68 (20-87) and mean BMI 28 (15-51). The primary diagnosis was cancer (n = 173), and there were no baseline differences between the groups. CO2 conditioning significantly decreased the incidence of postoperative hypothermia (odds ratio 0.10, 95 % CI 0.04-0.23), with hypothermic patients found to be at increased risk of SSI (odds ratio 4.0, 95 % CI 1.25-12.9). Use of conditioned CO2 significantly decreased the incidence of SSI by 66 % (p = 0.04). The intervention group incurred costs of £155 less per patient. The incremental cost-effectiveness ratio was negative. CONCLUSION: CO2 conditioning during laparoscopic colorectal surgery is a safe, feasible and a cost-effective intervention. It improves the quality of surgical care relating to SSI and postoperative hypothermia.
Alexander J, Gildea L, Balog J, et al., 2016, A novel methodology for in vivo endoscopic phenotyping of colorectal cancer based on real-time analysis of the mucosal lipidome: a prospective observational study of the iKnife, Surgical Endoscopy and Other Interventional Techniques, Vol: 31, Pages: 1361-1370, ISSN: 1432-2218
Background:This pilot study assessed the diagnostic accuracy of rapid evaporative ionization mass spectrometry (REIMS) in colorectal cancer (CRC) and colonic adenomas.Methods:Patients undergoing elective surgical resection for CRC were recruited at St. Mary’s Hospital London and The Royal Marsden Hospital, UK. Ex vivo analysis was performed using a standard electrosurgery handpiece with aspiration of the electrosurgical aerosol to a Xevo G2-S iKnife QTof mass spectrometer (Waters Corporation). Histological examination was performed for validation purposes. Multivariate analysis was performed using principal component analysis and linear discriminant analysis in Matlab 2015a (Mathworks, Natick, MA). A modified REIMS endoscopic snare was developed (Medwork) and used prospectively in five patients to assess its feasibility during hot snare polypectomy.Results:Twenty-eight patients were recruited (12 males, median age 71, range 35–89). REIMS was able to reliably distinguish between cancer and normal adjacent mucosa (NAM) (AUC 0.96) and between NAM and adenoma (AUC 0.99). It had an overall accuracy of 94.4 % for the detection of cancer versus adenoma and an adenoma sensitivity of 78.6 % and specificity of 97.3 % (AUC 0.99) versus cancer. Long-chain phosphatidylserines (e.g., PS 22:0) and bacterial phosphatidylglycerols were over-expressed on cancer samples, while NAM was defined by raised plasmalogens and triacylglycerols expression and adenomas demonstrated an over-expression of ceramides. REIMS was able to classify samples according to tumor differentiation, tumor budding, lymphovascular invasion, extramural vascular invasion and lymph node micrometastases (AUC’s 0.88, 0.87, 0.83, 0.81 and 0.81, respectively). During endoscopic deployment, colonoscopic REIMS was able to detect target lipid species such as ceramides during hot snare polypectomy.Conclusion:REIMS demonstrates high diagnostic accuracy for tumor type and for established histological featur
Kinross J, muirhead L, alexander J, et al., 2016, iKnife: Rapid evaporative ionization mass spectrometry (REIMS) enables real-time chemical analysis of the mucosal lipidome for diagnostic and prognostic use in colorectal cancer, American Association of Cancer Research, Publisher: American Association for Cancer Research, ISSN: 1538-7445
Mirnezami R, Veselkov K, Strittmatter N, et al., 2016, Spatially resolved profiling of colorectal cancer lipid biochemistry via DESI imaging mass spectrometry to reveal morphology-dependent alterations in fatty acid metabolism, Annual Meeting of the American-Society-of-Clinical-Oncology (ASCO), Publisher: American Society of Clinical Oncology, ISSN: 0732-183X
Background: Lipid metabolic alterations are recognised as potential oncogenic triggers that promote malignant transformation. Here we performed spatially-resolved profiling of lipid signatures in colorectal cancer (CRC) tissue and matched healthy mucosa using desorption electrospray ionisation imaging mass spectrometry (DESI-MSI). The objectives of this study were to comprehensively define the CRC ‘lipidome’ and to assess lipid signatures in discrete histological regions-of-interest, specifically morphologically bland peri-tumoural epithelium (PT-e) and tumour stroma (T-s). Methods: Fresh frozen tissue sections from 42 patients with confirmed CRC were subjected to negative-ion mode DESI-MSI analysis. Mass spectra in the 200-1000 m/zrange were collated from CRC epithelium (CRC-e), PT-e, T-s and healthy tumour-remote epithelium (TR-e). Spectral signatures were subjected to multivariate analysis using a recursive maximum margin criterion (RMMC) algorithm operating in MATLAB. Results: Increased levels of long/very-long chain fatty acids (LCFA/VLCFA) were seen in CRC-e compared with TR-e(AUC = 0.99). Correspondingly, increased expression of lipogenic and elongase enzymes was found on IHC. Transmission electron microscopy was performed to evaluate peroxisomal distribution and morphology in CRC-e, as these organelles metabolise LCFA/VLCFA through β-oxidation, to negligibly low levels, in healthy cells. No discernible difference in peroxisomal distribution, abundance or structure was found between CRC-e and TR-e. PT-e demonstrated a lipid expression pattern almost identical to that of CRC-e, and markedly different from TR-e (AUC = 0.89). Conclusions: A shift towards increased LCFA/VLCFA production may be an important metabolic trait in CRC facilitated through upregulation of de novo lipogenesis and fatty acid elongation and concurrent impairment of peroxisomal β-oxidation. This phenotype was also observed in morphologically bland PT-e, suggesting that
Alexander JL, Scott A, Mroz A, et al., 2016, 91 Mass Spectrometry Imaging (MSI) of Microbiome-Metabolome Interactions in Colorectal Cancer, 2016 Digestive Diseases Week, Publisher: Elsevier, Pages: S23-S23, ISSN: 0016-5085
Alexander JL, Scott A, Mroz A, et al., 2016, Mass Spectrometry Imaging (MSI) of Microbiome-Metabolome Interactions in Colorectal Cancer, Digestive Disease Week (DDW), Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S23-S23, ISSN: 0016-5085
Kinross JM, muirhead L, takats Z, 2016, Precision surgery and surgical spectroscopy, Metabolic Phenotyping in Public and Personalised Healthcare, Editors: Nicholson, Darzi, Holmes
Surgical practice is largely based on 20th century principles, and little, if any, biological data is provided to the clinician either preoperatively or intraoperatively to assist in decision making. Therefore novel technologies are urgently required to deliver the vision of personalized health care in surgery. Metabolic phenotyping has distinct advantages over other “-omics” based technologies in surgery, as the analysis of thousands of metabolites is possible in near real time, and it is able to provide critical data on tissue phenotypes and on the functional biochemistry of surgical pathology during surgery. The inference is that surgeons, pathologists, oncologists, and physicians will be able to augment current clinical strategies with chemical analysis at the patient bedside or in the operating theater. This chapter explores emerging technologies in this field and provides practical applications of their use in several areas of surgery and perioperative care. Particular attention is paid to oncology and the application of ambient mass spectrometry technologies in this field.
Kinross JM, marchesi J, 2016, The Ageing Superorganism, Metabolic Phenotyping in Personalized and Public Healthcare, Editors: Nicholson, darzi, Holmes
The aging population represents one of the greatest health challenges of modern times. For the first time in history, those over the age of 60 years will outnumber children under the age of 5 years. If the quality of life and the health of this growing population are to be improved, a deeper understanding of the aging process is required. This chapter analyzes aging from a superorganism perspective; it analyzes the contribution of the vast numbers of bacteria that inhabit the gut and the impact they have on human health; it provides a systematic overview of how the “-omics” technologies are revolutionizing our understanding of the biology of aging; it assesses our current understanding of how health may be maintained in advanced aging; and it looks to the future applications of the “-omics” technologies in this area.
Kinross J, Marchesi JR, 2016, The aging superorganism, Metabolic Phenotyping in Personalized and Public Healthcare, Pages: 265-290, ISBN: 9780128003442
The aging population represents one of the greatest health challenges of modern times. For the first time in history, those over the age of 60 years will outnumber children under the age of 5 years. If the quality of life and the health of this growing population are to be improved, a deeper understanding of the aging process is required. This chapter analyzes aging from a superorganism perspective; it analyzes the contribution of the vast numbers of bacteria that inhabit the gut and the impact they have on human health; it provides a systematic overview of how the "-omics" technologies are revolutionizing our understanding of the biology of aging; it assesses our current understanding of how health may be maintained in advanced aging; and it looks to the future applications of the "-omics" technologies in this area.
Tekkis P, Tan E, Kontovounisios C, et al., 2015, Hand-sewn coloanal anastomosis for low rectal cancer: technique and long-term outcome, Colorectal Disease, Vol: 17, Pages: 1062-1070, ISSN: 1463-1318
AimThis study compared the operative outcome and long-term survival of three types of hand-sewn coloanal anastomosis (CAA) for low rectal cancer.MethodPatients presenting with low rectal cancer at a single centre between 2006 and 2014 were classified into three types of hand-sewn CAA: type 1 (supra-anal tumours undergoing transabdominal division of the rectum with transanal mucosectomy); type 2 (juxta-anal tumours, undergoing partial intersphincteric resection); and type 3 (intra-anal tumours, undergoing near-total intersphincteric resection with transanal mesorectal excision).ResultsSeventy-one patients with low rectal cancer underwent CAA: 17 type 1; 39 type 2; and 15 type 3. The median age of patients was 61.6 years, with a male/female ratio of 2:1. Neoadjuvant therapy was given to 56 (79%) patients. R0 resection was achieved in 69 (97.2%) patients. Adverse events occurred in 25 (35.2%) of the 71 patients with a higher complication rate in type 1 vs type 2 vs type 3 (47.1% vs 38.5% vs 13.3%, respectively; P = 0.035). Anastomotic separation was identified in six (8.5%) patients and pelvic haematoma/seroma in five (7%); two (8.3%) female patients developed a recto–vaginal fistula. Ten (14.1%) patients were indefinitely diverted, with a trend towards higher long-term anastomotic failure in type 1 vs type 2 vs type 3 (17.6% vs 15.5% vs 6.7%). The type of anastomosis did not influence the overall or disease-free survival.ConclusionCAA is a safe technique in which anorectal continuity can be preserved either as a primary restorative option in elective cases of low rectal cancer or as a salvage procedure following a failed stapled anastomosis with a less successful outcome in the latter. CAA has acceptable morbidity with good long-term survival in carefully selected patients.
Penney NC, Kinross J, Newton R, et al., 2015, The role of bile acids in reducing the metabolic complications of obesity after bariatric surgery: A systematic review, International Journal of Obesity, Vol: 39, Pages: 1565-1574, ISSN: 1476-5497
Background: Bariatric surgery is currently the most efficacious treatment for obesity and its associated metabolic co-morbidities, such as diabetes. The metabolic improvements occur through both weight-dependent and weight-independent mechanisms. Bile acids (BAs) have emerged as key signalling molecules that have a central role in modulating many of the physiological effects seen after bariatric surgery. This systematic review assesses the evidence from both human and animal studies for the role of BAs in reducing the metabolic complications of obesity following bariatric surgery.Methods: We conducted a systematic search of Medline and Embase databases to identify all articles investigating the role of BAs in mediating the metabolic changes observed following bariatric surgery in both animal and human studies. Boolean logic was used with relevant search terms, including the following MeSH terms: ‘bile acids and salts’, ‘bariatric surgery’, ‘metabolic surgery’, ‘gastrointestinal tract/surgery’ and ‘obesity/surgery’.Results: Following database searches (n=1197), inclusion from bibliography searches (n=2) and de-duplication (n=197), 1002 search results were returned. Of these, 132 articles were selected for full-text review, of which 38 articles were deemed relevant and included in the review. The findings support the effects of BAs on satiety, lipid and cholesterol metabolism, incretins and glucose homoeostasis, energy metabolism, gut microbiota and endoplasmic reticulum stress following bariatric surgery. Many of these metabolic effects are modulated through the BA receptors FXR and TGR5. We also explore a possible link between BAs and carcinogenesis following bariatric surgery.Conclusions: Overall there is good evidence to support the role of BAs in the metabolic effects of bariatric surgery through the above mechanisms. BAs could serve as a novel therapeutic pharmacological target for the treatment of obes
Marchesi JR, Adams DH, Fava F, et al., 2015, The gut microbiota and host health: a new clinical frontier, Gut, Vol: 65, Pages: 330-339, ISSN: 1468-3288
Over the last 10–15 years, our understanding of the composition and functions of the human gut microbiota has increased exponentially. To a large extent, this has been due to new ‘omic’ technologies that have facilitated large-scale analysis of the genetic and metabolic profile of this microbial community, revealing it to be comparable in influence to a new organ in the body and offering the possibility of a new route for therapeutic intervention. Moreover, it might be more accurate to think of it like an immune system: a collection of cells that work in unison with the host and that can promote health but sometimes initiate disease. This review gives an update on the current knowledge in the area of gut disorders, in particular metabolic syndrome and obesity-related disease, liver disease, IBD and colorectal cancer. The potential of manipulating the gut microbiota in these disorders is assessed, with an examination of the latest and most relevant evidence relating to antibiotics, probiotics, prebiotics, polyphenols and faecal microbiota transplantation.
Balog J, Kumar, Alexander J, et al., 2015, In vivo endoscopic tissue identification tool utilising Rapid Evaporative Ionization Mass Spectrometry (REIMS), Angewandte Chemie International Edition, Vol: 54, Pages: 11059-11062, ISSN: 1433-7851
Gastrointestinal cancers are a leading cause of mortality, accounting for 23 % of cancer-related deaths worldwide. In order to improve outcomes from these cancers, novel tissue characterization methods are needed to facilitate accurate diagnosis. Rapid evaporative ionization mass spectrometry (REIMS) is a technique developed for the in vivo classification of human tissue through mass spectrometric analysis of aerosols released during electrosurgical dissection. This ionization technique was further developed by utilizing surface induced dissociation and was integrated with an endoscopic polypectomy snare to allow in vivo analysis of the gastrointestinal tract. We tested the classification performance of this novel endoscopic REIMS method in vivo. It was shown to be capable of differentiating between healthy layers of the intestinal wall, cancer, and adenomatous polyps based on the REIMS fingerprint of each tissue type in vivo.
Muirhead L, Kinross JM, Preece R, et al., 2015, A PROSPECTIVE, OBSERVATIONAL STUDY OF SURGICAL AND ENDOSCOPIC RAPID EVAPORATIVE IONISATION MASS SPECTROMETRY (REIMS) FOR REAL TIME ANALYSIS OF THE COLONIC MUCOSAL LIPIDOME IN COLORECTAL CANCER, 2nd Digestive-Disorders-Federation Conference, Publisher: BMJ PUBLISHING GROUP, Pages: A50-A51, ISSN: 0017-5749
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Li J, Kinross JM, Posma JP, et al., 2015, COLONIC MICROBIOME-METABONOME NETWORK INTERACTIONS IN AFRICAN AMERICANS AND NATIVE AFRICANS: A PROSPECTIVE 2-WEEK FOOD EXCHANGE STUDY, 2nd Digestive-Disorders-Federation Conference, Publisher: BMJ PUBLISHING GROUP, Pages: A369-A369, ISSN: 0017-5749
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