Imperial College London
Portrait Picture

Dr James Kinross

Faculty of Medicine, Department of Surgery & Cancer

Reader in General Surgery
 
 
 
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Contact

 

+44 (0)20 3312 1947j.kinross

 
 
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Location

 

1029Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Diederen:2020:10.1038/s41598-020-75306-z,
author = {Diederen, K and Li, JV and Donachie, GE and de, Meij TG and de, Waart DR and Hakvoort, TBM and Kindermann, A and Wagner, J and Auyeung, V and Te, Velde AA and Heinsbroek, SEM and Benninga, MA and Kinross, J and Walker, AW and de, Jonge WJ and Seppen, J},
doi = {10.1038/s41598-020-75306-z},
journal = {Scientific Reports},
title = {Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn's disease},
url = {http://dx.doi.org/10.1038/s41598-020-75306-z},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A nutritional intervention, exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn's disease (CD). We characterized changes in the fecal microbiota and metabolome to identify the mechanism of EEN. Feces of 43 children were collected prior, during and after EEN. Microbiota and metabolites were analyzed by 16S rRNA gene amplicon sequencing and NMR. Selected metabolites were evaluated in relevant model systems. Microbiota and metabolome of patients with CD and controls were different at all time points. Amino acids, primary bile salts, trimethylamine and cadaverine were elevated in patients with CD. Microbiota and metabolome differed between responders and non-responders prior to EEN. EEN decreased microbiota diversity and reduced amino acids, trimethylamine and cadaverine towards control levels. Patients with CD had reduced microbial metabolism of bile acids that partially normalized during EEN. Trimethylamine and cadaverine inhibited intestinal cell growth. TMA and cadaverine inhibited LPS-stimulated TNF-alpha and IL-6 secretion by primary human monocytes. A diet rich in free amino acids worsened inflammation in the DSS model of intestinal inflammation. Trimethylamine, cadaverine, bile salts and amino acids could play a role in the mechanism by which EEN induces remission. Prior to EEN, microbiota and metabolome are different between responders and non-responders.
AU  - Diederen,K
AU  - Li,JV
AU  - Donachie,GE
AU  - de,Meij TG
AU  - de,Waart DR
AU  - Hakvoort,TBM
AU  - Kindermann,A
AU  - Wagner,J
AU  - Auyeung,V
AU  - Te,Velde AA
AU  - Heinsbroek,SEM
AU  - Benninga,MA
AU  - Kinross,J
AU  - Walker,AW
AU  - de,Jonge WJ
AU  - Seppen,J
DO  - 10.1038/s41598-020-75306-z
PY  - 2020///
SN  - 2045-2322
TI  - Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn's disease
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-020-75306-z
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33144591
UR  - http://hdl.handle.net/10044/1/85302
VL  - 10
ER  -
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