Imperial College London


Faculty of MedicineDepartment of Infectious Disease

Research Fellow



+44 (0)20 7594 3058j.macdonald Website




Sir Alexander Fleming BuildingSouth Kensington Campus





I am a synthetic biologist working on cell-free protein expression systems and computational protein design. I develop computational methods as well as working in the wet-lab.

Please visit my personal web page to learn more about my research:



Kopniczky MB, Canavan C, McClymont DW, et al., 2020, Cell-free protein synthesis as a prototyping platform for mammalian synthetic biology, Acs Synthetic Biology, Vol:9, ISSN:2161-5063, Pages:144-156

Freemont PS, Moore S, MacDonald J, et al., 2018, Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria, Proceedings of the National Academy of Sciences, Vol:115, ISSN:0027-8424, Pages:E4340-E4349

Freemont PS, Salih O, He S, et al., 2018, Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa, Structure, Vol:26, ISSN:0969-2126, Pages:329-336.e3

MacDonald JT, Siciliano V, 2017, Computational Sequence Design with R2oDNA Designer., Methods Mol Biol, Vol:1651, Pages:249-262


Appuswamy R, Brigand KL, Barbry P, et al., 2019, OligoArchive: Using DNA in the DBMS storage hierarchy, CIDR 2019

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