Imperial College London
Alternate

ProfessorJulianMarchesi

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Digestive Health
 
 
 
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Contact

 

+44 (0)20 3312 6197j.marchesi

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Baker:2021:10.1016/j.ygeno.2021.08.007,
author = {Baker, LM and Davies, TS and Masetti, G and Hughes, TR and Marchesi, JR and Jack, AA and Joyce, TSC and Allen, MD and Plummer, SF and Michael, DR and Ramanathan, G and Del, Sol R and Facey, PD},
doi = {10.1016/j.ygeno.2021.08.007},
journal = {Genomics},
pages = {4028--4038},
title = {A genome guided evaluation of the Lab4 probiotic consortium},
url = {http://dx.doi.org/10.1016/j.ygeno.2021.08.007},
volume = {113},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In this study, we present the draft genome sequences of the Lab4 probiotic consortium using whole genome sequencing. Draft genome sequences were retrieved and deposited for each of the organisms; PRJNA559984 for B. bifidum CUL20, PRJNA482335 for Lactobacillus acidophilus CUL60, PRJNA482434 for Lactobacillus acid. Probiogenomic in silico analyses confirmed existing taxonomies and identified the presence putative gene sequences that were functionally related to the performance of each organism during in vitro assessments of bile and acid tolerability, adherence to enterocytes and susceptibility to antibiotics. Predictions of genomic stability identified no significant risk of horizontal gene transfer in any of the Lab4 strains and the absence of both antibiotic resistance and virulence genes. These observations were supported by the outcomes of acute phase and repeat dose tolerability studies in Wistar rats where challenge with high doses of Lab4 did not result in any mortalities, clinical/histopathological abnormalities nor indications of systemic toxicity. Detection of increased numbers of lactobacilli and bifidobacteria in the faeces of supplemented rats implied an ability to survive transit through the gastrointestinal tract and/or impact upon the intestinal microbiota composition. In summary, this study provides in silico, in vitro and in vivo support for probiotic functionality and the safety of the Lab4 consortium.
AU  - Baker,LM
AU  - Davies,TS
AU  - Masetti,G
AU  - Hughes,TR
AU  - Marchesi,JR
AU  - Jack,AA
AU  - Joyce,TSC
AU  - Allen,MD
AU  - Plummer,SF
AU  - Michael,DR
AU  - Ramanathan,G
AU  - Del,Sol R
AU  - Facey,PD
DO  - 10.1016/j.ygeno.2021.08.007
EP  - 4038
PY  - 2021///
SN  - 0888-7543
SP  - 4028
TI  - A genome guided evaluation of the Lab4 probiotic consortium
T2  - Genomics
UR  - http://dx.doi.org/10.1016/j.ygeno.2021.08.007
UR  - https://www.sciencedirect.com/science/article/pii/S0888754321003141?via%3Dihub
UR  - http://hdl.handle.net/10044/1/91437
VL  - 113
ER  -
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