Imperial College London
Dr James McKenzie

DrJamesMcKenzie

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Research Associate
 
 
 
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Contact

 

j.mckenzie

 
 
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Location

 

E311Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tillner:2016:10.1021/acs.analchem.6b00345,
author = {Tillner, J and McKenzie, JS and Jones, EA and Speller, AV and Walsh, JL and Veselkov, KA and Bunch, J and Takats, Z and Gilmore, IS},
doi = {10.1021/acs.analchem.6b00345},
journal = {Analytical Chemistry},
pages = {4808--4816},
title = {Investigation of the Impact of Desorption Electrospray Ionization Sprayer Geometry on Its Performance in Imaging of Biological Tissue.},
url = {http://dx.doi.org/10.1021/acs.analchem.6b00345},
volume = {88},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In this study, the impact of sprayer design and geometry on performance in desorption electrospray ionization mass spectrometry (DESI-MS) is assessed, as the sprayer is thought to be a major source of variability. Absolute intensity repeatability, spectral composition, and classification accuracy for biological tissues are considered. Marked differences in tissue analysis performance are seen between the commercially available and a lab-built sprayer. These are thought to be associated with the geometry of the solvent capillary and the resulting shape of the primary electrospray. Experiments with a sprayer with a fixed solvent capillary position show that capillary orientation has a crucial impact on tissue complex lipid signal and can lead to an almost complete loss of signal. Absolute intensity repeatability is compared for five lab-built sprayers using pork liver sections. Repeatability ranges from 1 to 224% for individual sprayers and peaks of different spectral abundance. Between sprayers, repeatability is 16%, 9%, 23%, and 34% for high, medium, low, and very low abundance peaks, respectively. To assess the impact of sprayer variability on tissue classification using multivariate statistical tools, nine human colorectal adenocarcinoma sections are analyzed with three lab-built sprayers, and classification accuracy for adenocarcinoma versus the surrounding stroma is assessed. It ranges from 80.7 to 94.5% between the three sprayers and is 86.5% overall. The presented results confirm that the sprayer setup needs to be closely controlled to obtain reliable data, and a new sprayer setup with a fixed solvent capillary geometry should be developed.
AU  - Tillner,J
AU  - McKenzie,JS
AU  - Jones,EA
AU  - Speller,AV
AU  - Walsh,JL
AU  - Veselkov,KA
AU  - Bunch,J
AU  - Takats,Z
AU  - Gilmore,IS
DO  - 10.1021/acs.analchem.6b00345
EP  - 4816
PY  - 2016///
SN  - 0003-2700
SP  - 4808
TI  - Investigation of the Impact of Desorption Electrospray Ionization Sprayer Geometry on Its Performance in Imaging of Biological Tissue.
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.6b00345
UR  - http://hdl.handle.net/10044/1/32459
VL  - 88
ER  -
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