Imperial College London

Emeritus ProfessorJeremyNicholson

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry



+44 (0)20 7594 3195j.nicholson Website




Ms Wendy Torto +44 (0)20 7594 3225




Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus






BibTex format

author = {Jimenez, B and Holmes, E and Heude, C and Tolson, RFM and Harvey, N and Lodge, SL and Chetwynd, AJ and Cannet, C and Fang, F and Pearce, JTM and Lewis, MR and Viant, MR and Lindon, JC and Spraul, M and Schaefer, H and Nicholson, JK},
doi = {10.1021/acs.analchem.8b02412},
journal = {Analytical Chemistry},
pages = {11962--11971},
title = {Quantitative lipoprotein subclass and low molecular weight metabolite analysis in human serum and plasma by 1H NMR spectroscopy in a multilaboratory trial},
url = {},
volume = {90},
year = {2018}

RIS format (EndNote, RefMan)

AB - We report an extensive 600 MHz NMR trial of a quantitative lipoprotein and small molecule measurements in human blood serum and plasma. Five centers with eleven 600 MHz NMR spectrometers were used to analyze 98 samples including: 20 QCs, 37 commercially sourced, paired serum and plasma samples and 2 National Institute of Science and Technology, NIST, reference material 1951c replicates. Samples were analyzed using rigorous protocols for sample preparation and experimental acquisition. A commercial lipoprotein subclass analysis was used to quantify 105 lipoprotein subclasses and 24 low molecular weight metabolites from the nuclear magnetic resonance, NMR, spectra. For all spectrometers, the instrument specific variance in measuring internal quality controls, QCs, was lower than the percentage described by the National Cholesterol Education Program, NCEP, criteria for lipid testing (triglycerides<2.7%, cholesterol<2.8%; LDL-cholesterol<2.8%; HDL-cholesterol<2.3%), showing exceptional reproducibility for direct quantitation of lipoproteins in both matrices. The average RSD for the 105 lipoprotein parameters in the 11 instruments was 4.6% and 3.9% for the two NIST samples while it was 38% and 40% for the 37 commercially sourced plasmas and sera, respectively, showing negligible analytical compared to biological variation. The coefficient of variance, CV, obtained for the quantification of the small molecules across the 11 spectrometers was below 15% for 20 out of the 24 metabolites analyzed. This study provides further evidence of the suitability of NMR for high-throughput lipoprotein subcomponent analysis and small molecule quantitation with the exceptional reproducibility required for clinical and other regulatory settings.
AU - Jimenez,B
AU - Holmes,E
AU - Heude,C
AU - Tolson,RFM
AU - Harvey,N
AU - Lodge,SL
AU - Chetwynd,AJ
AU - Cannet,C
AU - Fang,F
AU - Pearce,JTM
AU - Lewis,MR
AU - Viant,MR
AU - Lindon,JC
AU - Spraul,M
AU - Schaefer,H
AU - Nicholson,JK
DO - 10.1021/acs.analchem.8b02412
EP - 11971
PY - 2018///
SN - 0003-2700
SP - 11962
TI - Quantitative lipoprotein subclass and low molecular weight metabolite analysis in human serum and plasma by 1H NMR spectroscopy in a multilaboratory trial
T2 - Analytical Chemistry
UR -
UR -
UR -
VL - 90
ER -