Imperial College London

Emeritus ProfessorJeremyNicholson

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry



+44 (0)20 7594 3195j.nicholson Website




Ms Wendy Torto +44 (0)20 7594 3225




Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus






BibTex format

author = {McGill, D and Chekmeneva, E and Lindon, J and Takats, Z and Nicholson, J},
doi = {10.1039/C8FD00220G},
journal = {Faraday Discussions},
pages = {395--416},
title = {Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine},
url = {},
volume = {218},
year = {2019}

RIS format (EndNote, RefMan)

AB - Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artifical urine mixtures, which produce unique retention profiles useful to metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.
AU - McGill,D
AU - Chekmeneva,E
AU - Lindon,J
AU - Takats,Z
AU - Nicholson,J
DO - 10.1039/C8FD00220G
EP - 416
PY - 2019///
SN - 1359-6640
SP - 395
TI - Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine
T2 - Faraday Discussions
UR -
UR -
VL - 218
ER -