Imperial College London

Emeritus ProfessorJeremyNicholson

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3195j.nicholson Website

 
 
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Assistant

 

Ms Wendy Torto +44 (0)20 7594 3225

 
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Location

 

Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nicholson:1987:10.1007/978-3-0348-6784-9_12,
author = {Nicholson, JK and Sadler, PJ and Vasák, M},
doi = {10.1007/978-3-0348-6784-9_12},
journal = {Experientia Suppl},
pages = {191--201},
title = {Probing the reactivity of the zinc and cadmium ions bound to rabbit liver metallothioneins with EDTA.},
url = {http://dx.doi.org/10.1007/978-3-0348-6784-9_12},
volume = {52},
year = {1987}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Reactions of native and reconstituted rabbit liver metallothioneins (MT's) with excess EDTA at pH 7 were studied by 1H NMR, electronic absorption and atomic absorption spectroscopy. There was a reasonable agreement between the amounts of Zn2+ and Cd2+ removed as determined by the various methods. Specific structural changes accompanying metal removal were observable by NMR. Most of the Zn2+ in Zn7-MT, reacted rapidly with EDTA. One to two g-ions of Zn2+ in native Cd,Zn-MT's were labile, but none of the Cd2+ was extracted rapidly by EDTA. The cadmium protein Cd7-MT1, reconstituted via the low pH-apothionein method, appeared to contain EDTA-reactive Cd2+ bound in a different manner to either Zn2+ or Cd2+ in the native protein.
AU - Nicholson,JK
AU - Sadler,PJ
AU - Vasák,M
DO - 10.1007/978-3-0348-6784-9_12
EP - 201
PY - 1987///
SN - 0071-335X
SP - 191
TI - Probing the reactivity of the zinc and cadmium ions bound to rabbit liver metallothioneins with EDTA.
T2 - Experientia Suppl
UR - http://dx.doi.org/10.1007/978-3-0348-6784-9_12
UR - https://www.ncbi.nlm.nih.gov/pubmed/2959505
VL - 52
ER -