Imperial College London

Emeritus ProfessorJeremyNicholson

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3195j.nicholson Website

 
 
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Assistant

 

Ms Wendy Torto +44 (0)20 7594 3225

 
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Location

 

Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mirnezami:2014:10.1016/j.molonc.2013.08.010,
author = {Mirnezami, R and Spagou, K and Vorkas, PA and Lewis, MR and Kinross, J and Want, E and Shion, H and Goldin, RD and Darzi, A and Takats, Z and Holmes, E and Cloarec, O and Nicholson, JK},
doi = {10.1016/j.molonc.2013.08.010},
journal = {Molecular Oncology},
pages = {39--49},
title = {Chemical mapping of the colorectal cancer microenvironment via MALDI imaging mass spectrometry (MALDI-MSI) reveals novel cancer-associated field effects},
url = {http://dx.doi.org/10.1016/j.molonc.2013.08.010},
volume = {8},
year = {2014}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Matrixassisted laser desorption ionisation imaging mass spectrometry (MALDIMSI) is a rapidly advancing technique for intact tissue analysis that allows simultaneous localisation and quantification of biomolecules in different histological regions of interest. This approach can potentially offer novel insights into tumour microenvironmental (TME) biochemistry. In this study we employed MALDIMSI to evaluate fresh frozen sections of colorectal cancer (CRC) tissue and adjacent healthy mucosa obtained from 12 consenting patients undergoing surgery for confirmed CRC. Specifically, we sought to address three objectives: (1) To identify biochemical differences between different morphological regions within the CRC TME; (2) To characterise the biochemical differences between cancerous and healthy colorectal tissue using MALDIMSI; (3) To determine whether MALDIMSI profiling of tumouradjacent tissue can identify novel metabolic ‘field effects’ associated with cancer. Our results demonstrate that CRC tissue harbours characteristic phospholipid signatures compared with healthy tissue and additionally, different tissue regions within the CRC TME reveal distinct biochemical profiles. Furthermore we observed biochemical differences between tumouradjacent and tumourremote healthy mucosa. We have referred to this ‘field effect’, exhibited by the tumour locale, as canceradjacent metaboplasia (CAM) and this finding builds on the established concept of field cancerisation.
AU - Mirnezami,R
AU - Spagou,K
AU - Vorkas,PA
AU - Lewis,MR
AU - Kinross,J
AU - Want,E
AU - Shion,H
AU - Goldin,RD
AU - Darzi,A
AU - Takats,Z
AU - Holmes,E
AU - Cloarec,O
AU - Nicholson,JK
DO - 10.1016/j.molonc.2013.08.010
EP - 49
PY - 2014///
SN - 1574-7891
SP - 39
TI - Chemical mapping of the colorectal cancer microenvironment via MALDI imaging mass spectrometry (MALDI-MSI) reveals novel cancer-associated field effects
T2 - Molecular Oncology
UR - http://dx.doi.org/10.1016/j.molonc.2013.08.010
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000331510100005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.molonc.2013.08.010
UR - http://hdl.handle.net/10044/1/77926
VL - 8
ER -