Imperial College London

Emeritus ProfessorJeremyNicholson

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3195j.nicholson Website

 
 
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Assistant

 

Ms Wendy Torto +44 (0)20 7594 3225

 
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Location

 

Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ashrafian:2014:10.1021/pr400748f,
author = {Ashrafian, H and Li, J and Spagou, K and Harling, L and Masson, P and Darzi, A and Nicholson, J and Holmes, E and Athanasiou, T},
doi = {10.1021/pr400748f},
journal = {Journal of Proteome Research},
pages = {570--580},
title = {Bariatric surgery modulates circulating and cardiac metabolites},
url = {http://dx.doi.org/10.1021/pr400748f},
volume = {13},
year = {2014}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Bariatric procedures such as the Roux-en-Y gastric bypass (RYGB) operation offer profound metabolic enhancement in addition to their well-recognized weight loss effects. They are associated with significant reduction in cardiovascular disease risk and mortality, which suggests a surgical modification on cardiac metabolism. Metabolic phenotyping of the cardiac tissue and plasma postsurgery may give insight into cardioprotective mechanisms. The aim of the study was to compare the metabolic profiles of plasma and heart tissue extracts from RYGB- and sham-operated Wistar rats to identify the systemic and cardiac signature of metabolic surgery. A total of 27 male Wistar rats were housed individually for a week and subsequently underwent RYGB (n = 13) or sham (n = 14) operation. At week 8 postoperation, a total of 27 plasma samples and 16 heart tissue samples (8 RYGB; 8 Sham) were collected from animals and analyzed using 1H nuclear magnetic resonance (NMR) spectroscopy and ultra performance liquid chromatography (UPLC-MS) to characterize the global metabolite perturbation induced by RYGB operation. Plasma bile acids, phosphocholines, amino acids, energy-related metabolites, nucleosides and amine metabolites, and cardiac glycogen and amino acids were found to be altered in the RYGB operated group. Correlation networks were used to identify metabolite association. The metabolic phenotype of this bariatric surgical model inferred systematic change in both myocardial and systemic activity post surgery. The altered metabolic profile following bariatric surgery reflects an enhancement of cardiac energy metabolism through TCA cycle intermediates, cardiorenal protective activity, and biochemical caloric restriction. These surgically induced metabolic shifts identify some of the potential mechanisms that contribute toward bariatric cardioprotection through gut microbiota ecological fluxes and an enterocardiac axis to shield against metabolic syndrome of cardiac dysfunction.
AU - Ashrafian,H
AU - Li,J
AU - Spagou,K
AU - Harling,L
AU - Masson,P
AU - Darzi,A
AU - Nicholson,J
AU - Holmes,E
AU - Athanasiou,T
DO - 10.1021/pr400748f
EP - 580
PY - 2014///
SN - 1535-3893
SP - 570
TI - Bariatric surgery modulates circulating and cardiac metabolites
T2 - Journal of Proteome Research
UR - http://dx.doi.org/10.1021/pr400748f
UR - http://hdl.handle.net/10044/1/60538
VL - 13
ER -