Imperial College London

Emeritus ProfessorJeremyNicholson

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry



+44 (0)20 7594 3195j.nicholson Website




Ms Wendy Torto +44 (0)20 7594 3225




Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus






BibTex format

author = {Jackson, FL and Georgakopoulou, N and Kaluarachchi, M and Kyriakides, M and Andreas, N and Przysiezna, N and Hyde, M and Modi, N and Nicholson, J and Wijeyesekera, A and Holmes, E},
doi = {10.1021/acs.jproteome.6b00234},
journal = {Journal of Proteome Research},
pages = {3432--3440},
title = {Development of a pipeline for exploratory metabolic profiling of infant urine},
url = {},
volume = {15},
year = {2016}

RIS format (EndNote, RefMan)

AB - Numerous metabolic profiling pipelines have been developed to characterize the composition ofhuman biofluids and tissues, the vast majority of these being for studies in adults. To accommodatelimited sample volume and to take into account the compositional differences between adult andinfant biofluids, we developed and optimized sample handling and analytical procedures for studyingurine from newborns. A robust pipeline for metabolic profiling using NMR spectroscopy wasestablished, encompassing sample collection, preparation, spectroscopic measurement andcomputational analysis. Longitudinal samples were collected from five infants from birth until 14months of age. Methods of extraction, effects of freezing and sample dilution were assessed andurinary contaminants from breakdown of polymers in a range of diapers and cotton wool balls wereidentified and compared, including propylene glycol, acrylic acid and tert-butanol. Finally,assessment of urinary profiles obtained over the first few weeks of life revealed a dramatic change in composition, with concentrations of phenols, amino acids and betaine altering systematically overthe first few months of life. Therefore, neonatal samples require more stringent standardization ofexperimental design, sample handling and analysis compared to adult samples in order toaccommodate the variability and limited sample volume.
AU - Jackson,FL
AU - Georgakopoulou,N
AU - Kaluarachchi,M
AU - Kyriakides,M
AU - Andreas,N
AU - Przysiezna,N
AU - Hyde,M
AU - Modi,N
AU - Nicholson,J
AU - Wijeyesekera,A
AU - Holmes,E
DO - 10.1021/acs.jproteome.6b00234
EP - 3440
PY - 2016///
SN - 1535-3907
SP - 3432
TI - Development of a pipeline for exploratory metabolic profiling of infant urine
T2 - Journal of Proteome Research
UR -
UR -
VL - 15
ER -