Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
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Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Donderis:2017:10.1371/journal.ppat.1006581,
author = {Donderis, J and Bowring, J and Maiques, E and Ciges-Tomas, JR and Alite, C and Mehmedov, I and Tormo-Mas, MA and Penades, JR and Marina, A},
doi = {10.1371/journal.ppat.1006581},
journal = {PLoS Pathogens},
title = {Convergent evolution involving dimeric and trimeric dUTPases in pathogenicity island mobilization},
url = {http://dx.doi.org/10.1371/journal.ppat.1006581},
volume = {13},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The dUTPase (Dut) enzymes, encoded by almost all free-living organisms and some viruses, prevent the misincorporation of uracil into DNA. We previously proposed that trimeric Duts are regulatory proteins involved in different cellular processes; including the phage-mediated transfer of the Staphylococcus aureus pathogenicity island SaPIbov1. Recently, it has been shown that the structurally unrelated dimeric Dut encoded by phage NM1 is similarly able to mobilize SaPIbov1, suggesting dimeric Duts could also be regulatory proteins. How this is accomplished remains unsolved. Here, using in vivo, biochemical and structural approaches, we provide insights into the signaling mechanism used by the dimeric Duts to induce the SaPIbov1 cycle. As reported for the trimeric Duts, dimeric Duts contain an extremely variable region, here named domain VI, which is involved in the regulatory capacity of these enzymes. Remarkably, our results also show that the dimeric Dut signaling mechanism is modulated by dUTP, as with the trimeric Duts. Overall, our results demonstrate that although unrelated both in sequence and structure, dimeric and trimeric Duts control SaPI transfer by analogous mechanisms, representing a fascinating example of convergent evolution. This conserved mode of action highlights the biological significance of Duts as regulatory molecules.
AU  - Donderis,J
AU  - Bowring,J
AU  - Maiques,E
AU  - Ciges-Tomas,JR
AU  - Alite,C
AU  - Mehmedov,I
AU  - Tormo-Mas,MA
AU  - Penades,JR
AU  - Marina,A
DO  - 10.1371/journal.ppat.1006581
PY  - 2017///
SN  - 1553-7366
TI  - Convergent evolution involving dimeric and trimeric dUTPases in pathogenicity island mobilization
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1006581
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000411968300023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/80401
VL  - 13
ER  -
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