Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
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Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Fillol-Salom:2019:10.1371/journal.ppat.1007888,
author = {Fillol-Salom, A and Alsaadi, A and de, Sousa JAM and Zhong, L and Foster, KR and Rocha, EPC and Penades, JR and Ingmer, H and Haaber, J},
doi = {10.1371/journal.ppat.1007888},
journal = {PLoS Pathogens},
title = {Bacteriophages benefit from generalized transduction},
url = {http://dx.doi.org/10.1371/journal.ppat.1007888},
volume = {15},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Temperate phages are bacterial viruses that as part of their life cycle reside in the bacterial genome as prophages. They are found in many species including most clinical strains of the human pathogens, Staphylococcus aureus and Salmonella enterica serovar Typhimurium. Previously, temperate phages were considered as only bacterial predators, but mounting evidence point to both antagonistic and mutualistic interactions with for example some temperate phages contributing to virulence by encoding virulence factors. Here we show that generalized transduction, one type of bacterial DNA transfer by phages, can create conditions where not only the recipient host but also the transducing phage benefit. With antibiotic resistance as a model trait we used individual-based models and experimental approaches to show that antibiotic susceptible cells become resistant to both antibiotics and phage by i) integrating the generalized transducing temperate phages and ii) acquiring transducing phage particles carrying antibiotic resistance genes obtained from resistant cells in the environment. This is not observed for non-generalized transducing temperate phages, which are unable to package bacterial DNA, nor for generalized transducing virulent phages that do not form lysogens. Once established, the lysogenic host and the prophage benefit from the existence of transducing particles that can shuffle bacterial genes between lysogens and for example disseminate resistance to antibiotics, a trait not encoded by the phage. This facilitates bacterial survival and leads to phage population growth. We propose that generalized transduction can function as a mutualistic trait where temperate phages cooperate with their hosts to survive in rapidly-changing environments. This implies that generalized transduction is not just an error in DNA packaging but is selected for by phages to ensure their survival.
AU  - Fillol-Salom,A
AU  - Alsaadi,A
AU  - de,Sousa JAM
AU  - Zhong,L
AU  - Foster,KR
AU  - Rocha,EPC
AU  - Penades,JR
AU  - Ingmer,H
AU  - Haaber,J
DO  - 10.1371/journal.ppat.1007888
PY  - 2019///
SN  - 1553-7366
TI  - Bacteriophages benefit from generalized transduction
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1007888
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000478663700013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/80421
VL  - 15
ER  -
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