Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
//

Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
//

Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
//

Location

 

Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Gallego:2019:10.1016/j.molcel.2019.01.025,
author = {Gallego, del Sol F and Penades, JR and Marina, A},
doi = {10.1016/j.molcel.2019.01.025},
journal = {Molecular Cell},
pages = {59--72.e3},
title = {Deciphering the molecular mechanism underpinning phage arbitrium communication systems},
url = {http://dx.doi.org/10.1016/j.molcel.2019.01.025},
volume = {74},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bacillus phages use a communication system, termed “arbitrium,” to coordinate lysis-lysogeny decisions. Arbitrium communication is mediated by the production and secretion of a hexapeptide (AimP) during lytic cycle. Once internalized, AimP reduces the expression of the negative regulator of lysogeny, AimX, by binding to the transcription factor, AimR, promoting lysogeny. We have elucidated the crystal structures of AimR from the Bacillus subtilis SPbeta phage in its apo form, bound to its DNA operator and in complex with AimP. AimR presents intrinsic plasticity, sharing structural features with the RRNPP quorum-sensing family. Remarkably, AimR binds to an unusual operator with a long spacer that interacts nonspecifically with the receptor TPR domain, while the HTH domain canonically recognizes two inverted repeats. AimP stabilizes a compact conformation of AimR that approximates the DNA-recognition helices, preventing AimR binding to the aimX promoter region. Our results establish the molecular basis of the arbitrium communication system.
AU  - Gallego,del Sol F
AU  - Penades,JR
AU  - Marina,A
DO  - 10.1016/j.molcel.2019.01.025
EP  - 72
PY  - 2019///
SN  - 1097-2765
SP  - 59
TI  - Deciphering the molecular mechanism underpinning phage arbitrium communication systems
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2019.01.025
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000463340200008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S1097276519300450?via%3Dihub
UR  - http://hdl.handle.net/10044/1/84175
VL  - 74
ER  -
Download