Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
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Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Neamah:2017:nar/gkx308,
author = {Neamah, MM and Mir-Sanchis, I and Lopez-Sanz, M and Acosta, S and Baquedano, I and Haag, AF and Marina, A and Ayora, S and Penades, JR},
doi = {nar/gkx308},
journal = {Nucleic Acids Research},
pages = {6507--6519},
title = {Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus},
url = {http://dx.doi.org/10.1093/nar/gkx308},
volume = {45},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - DNA-single strand annealing proteins (SSAPs) are recombinases frequently encoded in the genome of many bacteriophages. As SSAPs can promote homologous recombination among DNA substrates with an important degree of divergence, these enzymes are involved both in DNA repair and in the generation of phage mosaicisms. Here, analysing Sak and Sak4 as representatives of two different families of SSAPs present in phages infecting the clinically relevant bacterium Staphylococcus aureus, we demonstrate for the first time that these enzymes are absolutely required for phage reproduction. Deletion of the genes encoding these enzymes significantly reduced phage replication and the generation of infectious particles. Complementation studies revealed that these enzymes are required both in the donor (after prophage induction) and in the recipient strain (for infection). Moreover, our results indicated that to perform their function SSAPs require the activity of their cognate single strand binding (Ssb) proteins. Mutational studies demonstrated that the Ssb proteins are also required for phage replication, both in the donor and recipient strain. In summary, our results expand the functions attributed to the Sak and Sak4 proteins, and demonstrate that both SSAPs and Ssb proteins are essential for the life cycle of temperate staphylococcal phages.
AU  - Neamah,MM
AU  - Mir-Sanchis,I
AU  - Lopez-Sanz,M
AU  - Acosta,S
AU  - Baquedano,I
AU  - Haag,AF
AU  - Marina,A
AU  - Ayora,S
AU  - Penades,JR
DO  - nar/gkx308
EP  - 6519
PY  - 2017///
SN  - 0305-1048
SP  - 6507
TI  - Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkx308
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000403693000036&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/80398
VL  - 45
ER  -
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