Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
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Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2016:10.1038/srep26455,
author = {Li, X and Koc, C and Kuehner, P and Stierhof, Y-D and Krismer, B and Enright, MC and Penades, JR and Wolz, C and Stehle, T and Cambillau, C and Peschel, A and Xia, G},
doi = {10.1038/srep26455},
journal = {Scientific Reports},
title = {An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus},
url = {http://dx.doi.org/10.1038/srep26455},
volume = {6},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Despite the importance of phages in driving horizontal gene transfer (HGT) among pathogenic bacteria, the underlying molecular mechanisms mediating phage adsorption to S. aureus are still unclear. Phage 11 is a siphovirus with a high transducing efficiency. Here, we show that the tail protein Gp45 localized within the 11 baseplate. Phage 11 was efficiently neutralized by anti-Gp45 serum and its adsorption to host cells was inhibited by recombinant Gp45 in a dose-dependent manner. Flow cytometry analysis demonstrated that biotin-labelled Gp45 efficiently stained the wild-type S. aureus cell but not the double knockout mutant ΔtarM/S, which lacks both α- and β-O-GlcNAc residues on its wall teichoic acids (WTAs). Additionally, adsorption assays indicate that GlcNAc residues on WTAs and O-acetyl groups at the 6-position of muramic acid residues in peptidoglycan are essential components of the 11 receptor. The elucidation of Gp45-involved molecular interactions not only broadens our understanding of siphovirus-mediated HGT, but also lays the groundwork for the development of sensitive affinity-based diagnostics and therapeutics for S. aureus infection.
AU  - Li,X
AU  - Koc,C
AU  - Kuehner,P
AU  - Stierhof,Y-D
AU  - Krismer,B
AU  - Enright,MC
AU  - Penades,JR
AU  - Wolz,C
AU  - Stehle,T
AU  - Cambillau,C
AU  - Peschel,A
AU  - Xia,G
DO  - 10.1038/srep26455
PY  - 2016///
SN  - 2045-2322
TI  - An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep26455
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000376340200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/80395
VL  - 6
ER  -
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