Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
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Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Fernandez:2018:10.1038/s41598-018-31107-z,
author = {Fernandez, L and Gonzalez, S and Quiles-Puchalt, N and Gutierrez, D and Penades, JR and Garcia, P and Rodriguez, A},
doi = {10.1038/s41598-018-31107-z},
journal = {Scientific Reports},
title = {Lysogenization of Staphylococcus aureus RN450 by phages phi 11 and phi 80 alpha leads to the activation of the SigB regulon},
url = {http://dx.doi.org/10.1038/s41598-018-31107-z},
volume = {8},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Staphylococcus aureus is a major opportunistic pathogen that commonly forms biofilms on various biotic and abiotic surfaces. Also, most isolates are known to carry prophages in their genomes. With this in mind, it seems that acquiring a better knowledge of the impact of prophages on the physiology of S. aureus biofilm cells would be useful for developing strategies to eliminate this pathogen. Here, we performed RNA-seq analysis of biofilm cells formed by S. aureus RN450 and two derived strains carrying prophages 11 and 80α. The lysogenic strains displayed increased biofilm formation and production of the carotenoid pigment staphyloxanthin. These phenotypes could be partly explained by the differences in gene expression displayed by prophage-harboring strains, namely an activation of the alternative sigma factor (SigB) regulon and downregulation of genes controlled by the Agr quorum-sensing system, especially the decreased transcription of genes encoding dispersion factors like proteases. Nonetheless, spontaneous lysis of part of the population could also contribute to the increased attached biomass. Interestingly, it appears that the phage CI protein plays a role in orchestrating these phage-host interactions, although more research is needed to confirm this possibility. Likewise, future studies should examine the impact of these two prophages during the infection.
AU  - Fernandez,L
AU  - Gonzalez,S
AU  - Quiles-Puchalt,N
AU  - Gutierrez,D
AU  - Penades,JR
AU  - Garcia,P
AU  - Rodriguez,A
DO  - 10.1038/s41598-018-31107-z
PY  - 2018///
SN  - 2045-2322
TI  - Lysogenization of Staphylococcus aureus RN450 by phages phi 11 and phi 80 alpha leads to the activation of the SigB regulon
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-018-31107-z
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000442530000026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/80407
VL  - 8
ER  -
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